| GFP叶绿体转化烟草茎叶降解对土壤微生物数量及土壤酶活性的影响 |
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| 引用本文： | 余建平,吕月萍,刘佳莉,刘庆国,郭长虹.GFP叶绿体转化烟草茎叶降解对土壤微生物数量及土壤酶活性的影响[J].农业环境科学学报,2011,30(3). |
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| 作者姓名： | 余建平 吕月萍 刘佳莉 刘庆国 郭长虹 |
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| 作者单位： | 哈尔滨师范大学生命科学与技术学院,分子细胞遗传与遗传育种黑龙江省重点实验室,哈尔滨,150025 |
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| 摘 要： | 以GFP叶绿体转基因烟草及对照烟草(非转基因烟草)为材料,在实验室条件下研究了叶绿体转基因烟草及对照烟草(非转基因烟草)在茎叶降解过程中及完全降解后对土壤微生物主要类群(细菌、真菌、放线菌)的影响,并对相关的土壤酶活性进行了分析.结果表明,在烟草茎叶降解过程中及完全降解后:(1)叶绿体转基因烟草及对照烟草(非转基因烟草)对微生物的数量影响不显著;(2)土壤微生物总量相比为细菌>放线菌>真菌;(3)叶绿体转基因烟草茎叶降解对土壤酶活性没有显著影响;(4)GFP基因没有水平转移到土壤微生物基因组中.以上结果显示GFP叶绿体转化烟草茎叶降解对土壤微生物数量及土壤酶活性没有显著的影响.
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| 关 键 词： | 叶绿体转化烟草 根际微生物 茎叶降解 GFP基因 土壤酶 |
Effects of the Stem and Leaf Decomposition of GFP Transplastomic Tobacco on Microorganism Populations and Enzymes Activity in Soil |
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| YU Jian-ping,LV Yue-ping,LIU Jia-li,LIU Qing-guo,GUO Chang-hong.Effects of the Stem and Leaf Decomposition of GFP Transplastomic Tobacco on Microorganism Populations and Enzymes Activity in Soil[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2011,30(3). |
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| Authors: | YU Jian-ping LV Yue-ping LIU Jia-li LIU Qing-guo GUO Chang-hong |
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| Institution: | YU Jian-ping,LV Yue-ping,LIU Jia-li,LIU Qing-guo,GUO Chang-hong*(Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province,College of Life Science and Technology,Harbin Normal University,Harbin 150025,China) |
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| Abstract: | The development and use of genetically modified plants(GMPs) have been a topic of considerable public debate in recent years.And there are many studies addressing potential risks of GMP cultivation,most of which have addressed only aboveground effects.However,there are few studies that evaluate the effects of the residue decomposition of transplastomic tobacco on microorganism populations and enzymes activity in soil.To study the effect of the transplastomic tobacco residue on the soil ecological environmen... |
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| Keywords: | transplastpmic tobacco rhizosphere microbial stem and leaf decomposition GFP gene soil enzymes |
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