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Action de l'atrazine, du chlortoluron et du MCPA sur les teneurs en pyruvate, oxalacétate, cétoglutarate, glutamate et glutamine dans les feuilles et les racines de concombre (Cucumis sativus)
Authors:M DECLEIRE  W DE  CAT
Institution:Institut de Recherches Chimiques, Museumlaan 5, Tervuren, B 1980, Belgium
Abstract:Action of atrazine, chlortoluron and MCPA on the pyrovate, oxalacetate, cetoglutarate, glutamate and glutamine content in the leaves and roots of cucumber (Cucumis sativus) Lethal rates of atrazine, chlortoluron and MCPA were administered to 17-day old cucumber seedlings growing in a nutrient solution. The ketonic acid, glutamate and glutamine composition of the leaves and roots was determined after intervals of 1–4 days. Two days after application of photosynthesis-inhibiting herbicides, the ketoglutarate, oxaloacetate, pyruvate, glutamate and glutamine content of the leaves was 11–20%; 30–54%; 43–50%; 101–112% and 49–65% respectively, compared with the controls. After 4 days it was 16–22%; 31–56%; 26–42%; 71–75% and 180–205%. In the roots, the glutamate decreased in the same proportion as the ketoglutarate while the glutamine increased the first 2 days and decreased on the fourth day. The MCPA treatment did not modify the cetoglutarate concentration in the leaves but increased it from 300 to 800% in the roots. Simultaneous analysis of ketoglutarate and glutamate can be used to identify the nature of a herbicide treatment.
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