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甜瓜种质资源亲缘关系的RAPD标记分析
引用本文:张建农,赵建华,李计红.甜瓜种质资源亲缘关系的RAPD标记分析[J].西北农林科技大学学报(社会科学版),2005,33(12):115-120.
作者姓名:张建农  赵建华  李计红
作者单位:甘肃农业大学,农学院,甘肃,兰州,730070
基金项目:甘肃省科技攻关项目(2GS032-A41-001)
摘    要:采用RAPD标记方法,对22份甜瓜材料进行了品种遗传多样性分析。结果显示,22个引物共扩增 出189条DNA条带,其中143条表现出多态性,占总带数的75.66%。聚类分析结果表明,22个甜瓜品种(系)中首 先可分为薄皮甜瓜与厚皮甜瓜两大类,彼此亲缘关系最远;厚皮甜瓜中网纹甜瓜与厚皮甜瓜的其他类型又被分开; 再次一级的分类为河套蜜、皇后和剩余的厚皮甜瓜。对照田间表型观察结果可见,聚类分析结果与传统分类结果相 近。

关 键 词:甜瓜  亲缘关系  RAPD标记  聚类分析
文章编号:1671-9387(2005)12-0115-06
收稿时间:2005-07-18
修稿时间:2005年7月18日

Genetic relationship analysis of melon by RAPD marker
ZHANG Jian-nong,ZHAO Jian-hua,LI Ji-hong,CAO Zi-yi.Genetic relationship analysis of melon by RAPD marker[J].Journal of Northwest Sci-Tech Univ of Agr and,2005,33(12):115-120.
Authors:ZHANG Jian-nong  ZHAO Jian-hua  LI Ji-hong  CAO Zi-yi
Institution:(G’ansu agricultural university ,Lanzhou 730070,China)
Abstract:Twenty-two melon varieties were used to analyze genetic diversity by RAPD marker. Results showed that 189 DNA bands were amplfied with 22 random primers,of which 143 were polymorphic. An average proportion of polymorphic loci was 75.66%. Twenty-two varieties and inbreds were classified into two sub-groups ,which were thick-peel melon and thin-peel melon. The cantaloupe was far from others among thick-peel melons. And then hetaomi,huanghou ,and the rest melons were classified into sub-groups. This result approached the classification of the traditional method according to characters in field.
Keywords:melon  genetic relationship RAPD  cluster analysis
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