| 侧耳菌GH196木质纤维素降解酶系的研究 |
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| 引用本文： | 闵江马[,] 付时雨.侧耳菌GH196木质纤维素降解酶系的研究[J].林产化学与工业,2005,25(10):105-108. |
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| 作者姓名： | 闵江马[ ] 付时雨 |
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| 作者单位： | [1]中国科学院广州化学研究所,广东广州510650 [2]中国科学院研究生院,北京100039 [3]华南理工大学制浆造纸工程国家重点实验室,广东广州510640 |
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| 基金项目： | 国家自然科学基金资助项目（30270040）;广东省自然科学基金资助项目（32543） |
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| 摘 要： | 以一株从腐朽竹子上分离得到的侧耳菌（Pleurotus sp．）GH196为产酶菌，研究了其在静置和振荡两种液体培养方式下的木质纤维素降解酶系的产生。结果表明：在含有稻草和麦麸的液体培养基中生长，可以产生木聚糖酶、纤维素酶和依赖锰过氧化物酶-漆酶型（MnPLaccas型）木质素降解酶。振荡条件可以促进菌丝生长，有利于多糖水解酶的产生，其中以木聚糖酶的合成为主；150r／min振荡培养5d，木聚糖酶酶活达到44．7U／mL。
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| 关 键 词： | 侧耳菌 木聚糖酶 纤维素酶 木质素降解酶 |
| 收稿时间： | 03 15 2005 12:00AM |
LIGNOCELLULOSE-DEGRADING ENZYME SYSTEM OF PLEUROTUS SP. GH196 |
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| MIN Jiang-ma , FU Shi-yu.LIGNOCELLULOSE-DEGRADING ENZYME SYSTEM OF PLEUROTUS SP. GH196[J].Chemistry & Industry of Forest Products,2005,25(10):105-108. |
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| Authors: | MIN Jiang-ma FU Shi-yu |
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| Institution: | 1. Guangzhou Institute of Chemistry, Chinese Academy of Sciences, Guangzhou 510650, China; 2. Graduate School of Chinese Academy of Sciences,Beijing 100039, China; 3. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China |
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| Abstract: | To investigate the production of lignocellulose-degrading enzyme system from Pleurotus sp. GH196, isolated from a rotten bamboo, submerged fermentation of this fungus was performed under shaking and static conditions. The result showed that lignolytic enzymes produced by GH196 are composed of laccase and manganese peroxidase. Shaking could promote the mycelium growth of the fungus and synthesis of cellulase and xylanase, among them xylanase is the master polysaccharide hydrolase. The xylanase activity can reach 44.7 U/mL when GH196 was cultivated by using rice straw and wheat bran as compound carbon source under 150 r/min shaking condition. |
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| Keywords: | Pleurotus sp xylanase cellulase lignolytic enzyme |
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