| 22个毛木耳菌株ITS序列分析 |
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| 引用本文： | 贾定洪,王波,彭卫红,甘炳成,黄忠乾,郑林用.22个毛木耳菌株ITS序列分析[J].西南农业学报,2011,24(1). |
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| 作者姓名： | 贾定洪 王波 彭卫红 甘炳成 黄忠乾 郑林用 |
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| 作者单位： | 四川省农业科学院土壤肥料研究所,四川,成都,610066 |
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| 基金项目： | 四川省财政育种工程青年基金，公益行业专项，农业部948项目2006-G11 |
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| 摘 要： | 以22个不同来源的毛木耳菌株为材料,采用MEGA4.0软件对ITS序列进行分析,构建系统发育树.试验结果显示22个菌株的ITS序列长度为620 bp左右,GC含在50.6%～51.1%,被聚为5个分支,其中黄耳10号和781系统发育关系较近,共同聚在第一个分支,而琥珀聚在第二分支.ITS序列分析试验结果从系统发育角度反映出了研究菌株的遗传关系,是进行毛木耳菌株遗传分析及科学鉴定的重要工具.
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| 关 键 词： | 毛木耳 |
Analysis on 22 Auricularia polytricha Strains Using ITS Sequencing |
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| JIA Ding-hong,WANG Bo,PENG Wei-hong,GAN Bing-cheng,HUANG Zhong-qian,ZHENG Lin-yong.Analysis on 22 Auricularia polytricha Strains Using ITS Sequencing[J].Southwest China Journal of Agricultural Sciences,2011,24(1). |
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| Authors: | JIA Ding-hong WANG Bo PENG Wei-hong GAN Bing-cheng HUANG Zhong-qian ZHENG Lin-yong |
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| Institution: | JIA Ding-hong,WANG Bo,PENG Wei-hong,GAN Bing-cheng,HUANG Zhong-qian,ZHENG Lin-yong(Soil and Fertilizer Institute,Sichuan Academy of Agricultural Sciences,Sichuan Chengdu 610066,China) |
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| Abstract: | 22 Auricularia polytricha strains were studied using internal transcribed spacer(ITS) sequencing method and the phylogenetic tree was generated based on Kimura-2 parameters.Results showed that the ITS lengths of 22 Auricularia polytricha strains were about 620 bp,GC content ranged from 50.6% to 51.1 %,and all were clustered into 5 clades,among which,Huanger10# and 781 had a closer phylogenetic relationship and were clustered into the first clade,while Hupo into another the second clade.The phylogenetic rela... |
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| Keywords: | MEGA4 0 ITS |
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