| 猪伪狂犬病毒的增殖、纯化和鉴定 |
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| 引用本文： | 王大伟,李学伍,王丽,史西保.猪伪狂犬病毒的增殖、纯化和鉴定[J].河南农业科学,2010(11). |
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| 作者姓名： | 王大伟 李学伍 王丽 史西保 |
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| 作者单位： | 1. 河南科技大学,动物科技学院,河南,洛阳,471003;河南省农业科学院农业部动物免疫学重点开放实验室/河南省动物免疫学重点实验室,河南,郑州,450002
2. 河南科技大学,动物科技学院,河南,洛阳,471003 |
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| 摘 要： | 以伪狂犬病毒(PRV)活疫苗Bartha弱毒株接种于幼仓鼠肾细胞(BHK-21)使其增殖,通过PCR方法鉴定了病毒。以组织细胞半数感染剂量(TCID50)对病毒效价进行了评定,利用蔗糖密度梯度离心法纯化了病毒。结果表明,培养的病毒滴度为103.25TCID50/mL,纯化后的病毒经SDS-PAGE电泳显示,获得了较纯的蛋白条带。
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| 关 键 词： | 伪狂犬病毒 仓鼠肾细胞 差速离心 纯化 鉴定 |
Identification and Purification of Pseudorabies Virus Proliferated in BHK-21 Cells |
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| WANG Da-wei,LI Xue-wu,WANG Li,SHI Xi-bao.Identification and Purification of Pseudorabies Virus Proliferated in BHK-21 Cells[J].Journal of Henan Agricultural Sciences,2010(11). |
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| Authors: | WANG Da-wei LI Xue-wu WANG Li SHI Xi-bao |
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| Abstract: | Baby hamster kidney cells(BHK-21) were infected with the live vaccine strain Bartha of pseudorabies virus(PRV).The viral DNA was extracted from infected BHK cells and identified by PCR.The virus titer was determined by calculating median tissue culture infective dose(TCID50),and the results showed that the titer was about 103.25TCID50/mL.The virus was purified by sucrose density-gradient centrifugation and then analyzed by SDS-PAGE,which represented more pure protein bands of the virus. |
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| Keywords: | Pseudorabies virus(PRV) BHK-21 Differential centrifugation Purification Identification |
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