| 不同昆虫泛素延伸蛋白基因的生物信息学分析 |
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| 引用本文： | 强承魁,王胜永,凤舞剑,于玲雅,秦越华.不同昆虫泛素延伸蛋白基因的生物信息学分析[J].河南农业科学,2011,40(3):92-95. |
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| 作者姓名： | 强承魁 王胜永 凤舞剑 于玲雅 秦越华 |
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| 作者单位： | 1. 徐州生物工程职业技术学院,农林工程系,江苏,徐州,221006
2. 山东省植物保护总站,山东,济南,250100 |
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| 基金项目： | 徐州生物工程职业技术学院院级科研课题 |
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| 摘 要： | 采用生物信息学方法系统分析了甜菜夜蛾、菜粉蝶、烟夜蛾和黑腹果蝇泛素延伸蛋白(UBE)基因序列的遗传多样性、分子进化及其编码氨基酸序列的结构功能域和基序特点.结果显示,4种昆虫UBE基因序列的同源性较高(81.8%～99.7%),且遗传距离较近(0.003～0.199),均编码ubiquitin/L40 UBE.共检出8...
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| 关 键 词： | 泛素延伸蛋白 甜菜夜蛾 菜粉蝶 烟夜蛾 黑腹果蝇 生物信息学 |
Bioinformatic Analysis of Ubiquitin Extension Protein Genes from Different Insects |
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| QIANG Cheng-kui,WANG Sheng-yong,FENG Wu-jian,YU Ling-ya,QIN Yue-hua.Bioinformatic Analysis of Ubiquitin Extension Protein Genes from Different Insects[J].Journal of Henan Agricultural Sciences,2011,40(3):92-95. |
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| Authors: | QIANG Cheng-kui WANG Sheng-yong FENG Wu-jian YU Ling-ya QIN Yue-hua |
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| Institution: | 1(1.Department of Agriculture and Landscape Engineering,Xuzhou Bioengineering Technical College,Xuzhou 221006,China;2.Shandong Station of Plant Protection,Jinan 250100,China) |
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| Abstract: | Based on the published sequences of ubiquitin extension protein(UBE) genes of Spodoptera exigu,Pieris rapa,Helicoverpa assulta,Drosophila melanogaste in NCBI database,the genetic diversity and molecular evolution of the nucleotide sequences,as well as structural domains and motif characters of putative amino acid sequences were analyzed using the bioinformatics method.Results showed high identity(81.8%-99.7%) and short genetic distances(0.003-0.199)between the four UBE gene sequences,and all encoded ubiquitin/L40 UBE.During the genetic diversity analysis,81 polymorphic sites in total were detected from 4 UBE gene sequences and 4 haplotypes were sorted.Abundant genetic diversity and strong codon bias were found based on the haplotype diversity(Hd=1.000),the average number of nucleotide difference(K=45.883),the nucleotide diversity(Pi=0.11297),the nucleotide diversity of synonmyous sites(Pi(s)=0.41501),the nucleotide diversity of nonsynonmyous sites(Pi(a)= 0.02489),the effective number of codons(ENC=38.070-53.458<61),the codon bias index(CBI=0.437-0.729>0) and the scaled Chi-square(χ2=0.454-0.960).Each amino acid sequence deduced from its corresponding UBE gene contained one ubiquitin homologue and one Ribosomal/L40 domain,respectively,but signal peptide and transmembrane domain were not found.Fourteen motifs were detected among 4 amino acid sequences.Deduced amoino acid sequences from the Se-UBE gene and Dm-UBE gene contained 1 casein kinase Ⅱ phosphorylation site and 2 protein kinase C phosphorylation sites.Deduced amino acid sequences from the Pr-UBE gene and Ha-UBE gene both had 2 casein kinase Ⅱ phosphorylation sites and 2 protein kinase C phosphorylation sites.The result provided some basic information for further studying the evolution and expression characteristics of UBE genes as well as mechanism of insect viral infection. |
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| Keywords: | Ubiquitin extension protein Spodoptera exigu Pieris rapa Helicoverpa assulta Drosophila melanogaster Bioinformatics |
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