Serial invasive signal amplification reaction for genotyping permethrin-resistant (kdr-like) human head lice, Pediculus capitis |
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| Authors: | Hyo Jeong Kim Steven B Symington Si Hyeock Lee J Marshall Clark |
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| Institution: | aMolecular and Cellular Biology Program, University of Massachusetts, Amherst, MA 01003, USA;bSchool of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea;cDepartment of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA |
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| Abstract: | Permethrin resistance in the human head louse, Pediculus capitis De Geer (Anopulura: Pediculidae), has been reported worldwide, is associated with the knockdown phenotype, and elicits cross-resistance to DDT and the pyrethrins. Two point mutations, T929I and L932F, in the voltage-sensitive sodium channel α-subunit gene are responsible for permethrin resistance as a resistant haplotype (kdr-like). We have optimized a serial invasive signal amplification reaction (SISAR) protocol for the detection of these mutations using PCR amplified DNA fragments. SISAR distinguished all genotypes with high accuracy in a head louse population from Texas that was heterogeneous in terms of permethrin sensitivity. Using SISAR, resistance-conferring mutations are detected in a high throughput format, facilitating the efficient monitoring of permethrin resistance allele frequency in field populations. |
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| Keywords: | Serial invasive signal amplification reaction (SISAR) Genotyping Mutation detection Permethrin-resistant Human head lice |
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