| 羊同期发情腹腔镜输精最佳时间的研究 |
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| 引用本文： | 羊同期发情腹腔镜输精最佳时间的研究.羊同期发情腹腔镜输精最佳时间的研究[J].畜牧与饲料科学,2021,42(4):46-48. |
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| 作者姓名： | 羊同期发情腹腔镜输精最佳时间的研究 |
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| 作者单位： | 1.内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031;2.锡林郭勒职业学院,内蒙古 锡林浩特 026000;3.赤峰市巴林左旗乌兰达坝苏木动物防疫站,内蒙古 巴林左旗 025463;4.赤峰市巴林左旗畜牧工作站,内蒙古 巴林左旗 025450 |
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| 基金项目： | 内蒙古农牧业创新基金项目（2020CXJJM05） |
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| 摘 要： | 目的]确定羊同期发情腹腔镜输精的最佳时间。方法]选取繁殖机能正常、营养状况良好的适龄母羊,在试验母羊阴道内放置孕酮海绵栓10~14 d,撤栓时肌肉注射250~330 IU/只孕马血清促性腺激素（PMSG）。撤栓后12~24 h开始试情,早晚各1次。按撤栓后时间及发情时间不同分4组进行腹腔镜输精：1组为撤栓后46~50 h输精（n=138）,2组为撤栓后50~54 h输精（n=156）,3组为撤栓后54~58 h输精（n=275）,4组为羊发情后30~36 h输精（n=226）。输精后第40~45天进行B超检查,计算各组母羊受胎率。结果]发情后30~36 h输精的母羊（4组）受胎率最高,为97.79%,其次为撤栓后50~54 h输精的母羊（2组）,受胎率为97.44%,撤栓后46~50 h输精的母羊（1组）受胎率为91.30%;撤栓后54~58 h输精的母羊（3组）受胎率最低,为89.82%。结论]采用同期发情配合腹腔镜输精技术进行母羊人工授精,最佳输精时间为撤栓后50~54 h以及母羊发情后30~36 h。
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| 关 键 词： | 羊 同期发情 人工授精 腹腔镜输精 |
| 收稿时间： | 2020-08-25 |
Determination of Optimum Time of Laparoscopically Assisted Artificial Insemination in Combination with Estrus Synchronization in Sheep |
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| ZHAO Xia,MA Yue-jun,LI Yu-rong,LIU Bin,WU Tie-cheng,Wulanqiqige,Suyalatu,Wurentuya.Determination of Optimum Time of Laparoscopically Assisted Artificial Insemination in Combination with Estrus Synchronization in Sheep[J].Animal Husbandry and Feed Science,2021,42(4):46-48. |
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| Authors: | ZHAO Xia MA Yue-jun LI Yu-rong LIU Bin WU Tie-cheng Wulanqiqige Suyalatu Wurentuya |
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| Institution: | 1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;2. Xilin Gol Vocational College,Xilinhot 026000,China;3. Animal Epidemic Prevention Station of Wulandaba Sumu of Bairin Left Banner of Chifeng City,Bairin Left Banner 025463,China;4. Animal Husbandry Workstation of Bairin Left Banner of Chifeng City,Bairin Left Banner 025450,China |
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| Abstract: | Objective] To determine the optimum time of laparoscopically assisted artificial insemination in sheep treated with estrus synchronization. Method]The ewes with normal reproductive performance and good nutritional status were selected. The progesterone sponges were placed in vaginas of the ewes for 10-14 days, and 250-330 IU of pregnant mare serum gonadotropin （PMSG） were intramuscularly injected for each ewe when the sponge was removed. For each ewe, estrus detections were conducted twice 12-24 h after sponge removal, once in the morning and another in the evening. Four experimental groups were set according to the time after sponge removal or estrus time: group 1 （n=138） was laparoscopically inseminated 46-50 h after sponge removal, group 2 （n=156） was laparoscopically inseminated 50-54 h after sponge removal, group 3 （n=275） was laparoscopically inseminated 54-58 h after sponge removal, and group 4 （n=226） was laparoscopically inseminated 30-36 h after estrus. B-ultrosonic examination was conducted 40-45 days after insemination, and the conception rate of each group was calculated. Result] The highest conception rate （97.79%） was observed in the ewes laparoscopically inseminated 30-36 h after estrus （group 4）, followed by those laparoscopically inseminated 50-54 h after sponge removal （group 2）, which had a conception rate of 97.44%. The conception rate of the ewes laparoscopically inseminated 46-50 h after sponge removal （group 1） was 91.30%. The ewes laparoscopically inseminated 54-58 h after sponge removal （group 3） had the lowest conception rate （89.82%）. Conclusion]For laparoscopically assisted insemination of ewes in combination with estrus synchronization, the optimum insemination time is 50-54 h after progesterone sponge removal or 30-36 h after estrus. |
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| Keywords: | sheep estrus synchronization artificial insemination laparoscopically assisted insemination |
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