| 内蒙古锡林郭勒盟犊牛腹泻大肠杆菌的分离鉴定及药物敏感性试验 |
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| 引用本文： | 内蒙古锡林郭勒盟犊牛腹泻大肠杆菌的分离鉴定及药物敏感性试验.内蒙古锡林郭勒盟犊牛腹泻大肠杆菌的分离鉴定及药物敏感性试验[J].畜牧与饲料科学,2021,42(6):106-111. |
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| 作者姓名： | 内蒙古锡林郭勒盟犊牛腹泻大肠杆菌的分离鉴定及药物敏感性试验 |
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| 作者单位： | 1.内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031; 2.呼伦贝尔市新巴尔虎右旗动物疫病预防控制中心,内蒙古 新巴尔虎右旗 021300 |
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| 基金项目： | 内蒙古农牧业创新基金项目（2021CXJJM04）; 内蒙古自治区科技重大专项（2020ZD0006） |
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| 摘 要： | 目的] 确定内蒙古锡林郭勒盟多伦县某养殖场犊牛腹泻的致病菌,为选择敏感药物治疗该病提供依据。方法] 无菌采集腹泻犊牛肛拭子进行细菌培养鉴定、致病性试验及体外药物敏感试验。结果] 肛拭子经培养后的菌落形态一致,经表型鉴定、生理生化鉴定及16S rDNA鉴定确定分离菌为大肠杆菌;大肠杆菌分型引物鉴定分离菌株为ETEC型;且分离菌株可致小鼠死亡;分离菌株对氨苄西林、氨曲南、头孢唑啉、四环素、氧氟沙星、左氧氟沙星、复方新诺明等21种药物表现为耐药,对头孢哌酮、头孢曲松、克林霉素、多黏菌素B、米诺环素等10种药物表现为中度敏感,对头孢西丁、万古霉素、环丙沙星及呋喃妥因4种药物表现为敏感。结论] 该养殖场的犊牛腹泻是由大肠杆菌ETEC型所致,临床治疗过程中可使用环丙沙星。
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| 关 键 词： | 大肠杆菌 犊牛 腹泻 分离鉴定 |
| 收稿时间： | 2021-08-04 |
Isolation,Identification and Antimicrobial Sensitivity Test of Escherichia coli Associated with Calf Diarrhea in Xilin Gol League,Inner Mongolia |
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| WANG Na,DAI Ling-li,Wuyuntana,Gangtuoya,ZHAO Shi-hua,Dalaibaolige.Isolation,Identification and Antimicrobial Sensitivity Test of Escherichia coli Associated with Calf Diarrhea in Xilin Gol League,Inner Mongolia[J].Animal Husbandry and Feed Science,2021,42(6):106-111. |
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| Authors: | WANG Na DAI Ling-li Wuyuntana Gangtuoya ZHAO Shi-hua Dalaibaolige |
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| Institution: | 1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China; 2. Animal Disease Prevention and Control Center of New Barag Right Banner of Hulun Buir City,New Barag Right Banner 021300,China |
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| Abstract: | Objective] To determine the pathogenic bacteria causing calf diarrhea in a cattle farm in Duolun County, Xilin Gol League, Inner Mongolia, and to provide a basis for selecting sensitive drugs to treat this disease. Method] Anal swabs from calves with diarrhea were collected aseptically for bacterial isolation, identification, pathogenicity test and in vitro antimicrobial sensitivity test. Result] The bacterial strains isolated from the anal swab samples had the consistent colony morphology. The isolates were confirmed as Escherichia coli （E. coli） by phenotypic identification, physiological and biochemical identification and 16S rDNA gene sequencing identification. The isolates were molecularly identified as ETEC type by using the E. coli typing primers. In addition, the E. coli isolates could cause mice death. The E. coli isolats were resistant to 21 antimicrobial agents such as ampicillin, aztreonam, cefazolin, tetracycline, ofloxacin, levofloxacin and compound trimethoprim, were moderately sensitive to 10 antimicrobial agents such as cefoperazone, ceftriaxone, clindamycin, polymyxin B and minocycline, and were sensitive to 4 antimicrobial agents such as cefoxitin, vancomycin, ciprofloxacin and nitrofurantoin. Conclusion] The calf diarrhea in this cattle farm was caused by enterotoxigenic E. coli. Ciprofloxacin was recommended to be used in treatment of this disease. |
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| Keywords: | Escherichia coli calf diarrhea isolation and identification |
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