Morphological and molecular analysis of androgenetic, selfed and backcrossed plants produced from a Hordeum vulgare x H, bulbosum hybrid |
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| Authors: | M J gilpin R A pickering A G fautrier D L mcneil G szigat A M Hill R G Kynast |
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| Institution: | Planl Science Department, Lincoln University, P.O. Box 84, Canterbury, New Zealand;New Zealand Institute for Crop &Food Research Limited, Private Bag 4704, Christchureh, New Zealand;(present address) The Royal Veterinary and Agricultural University, Department of Plant Biology, 40 Thorvaldsensvej, DK;-1871 Frederiksberg C. Copenhagen, Denmark Mühlbergstraβe 6, D-18276 Gβlzow, Germany;Department of Plant Pathology, Kansas State University, Manhattan, KS 66506-5502, USA;Corresponding author |
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| Abstract: | Anther culture (AC) was carried out on a fertile triploid hybrid between Hordeum vulgare L. (cultivated barley) and H. bulbosum L, (bulbous barley grass) to determine whether AC-derived regenerants differed from progeny obtained through selfing and backcrossing. Chromosome counts were carried out on all plants and DNA was extracted from them to prepare Southern blots for molecular analysis. To identify true recombinants, the blots were probed with rye repetitive sequence probes (pSc119.1 and pScl19.2). which hybridize strongly and specifically to H. bulbosum DNA. Twenty probes that detect single- or low-copy sequences were hybridized with Southern blots containing restricted DNA extracted from 25 AC-derived plants, 11 selfed and six backcrossed progeny that showed hybridizations with pScll9. Although restriction fragment length polymorphisms (RFLPs) were only observed using probes that map to four of the possible 14 chromosome arms, an introgression associated with chromosome 6HS was frequently observed among plants derived from AC. selfing and backcrossing. Plants from AC differed from selfed and backcrossed progeny in their chromosome number; unique RFLP bands that were occasionally observed may indicate chromosomal rearrangements. |
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| Keywords: | Hordeum bulbosum H vulgare androgenesis gene introgression interspecific hybridization RFLP |
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