| H-Y单抗检测方法初探 |
| |
| 引用本文： | 牛树理,刘为敏,杭苏琴,冯紫云.H-Y单抗检测方法初探[J].中国畜牧杂志,2001,37(6):6-8. |
| |
| 作者姓名： | 牛树理 刘为敏 杭苏琴 冯紫云 |
| |
| 作者单位： | 南京农业大学动物科技学院, |
| |
| 摘 要： | 优化间接ELISA条件 ,提高灵敏度 ,建立检测H Y抗体的检测方法。利用BALB/c公鼠免疫同系母鼠 ,制备H Y单抗。PCR验证间接免疫荧光法鉴定胚胎性别准确率。结果表明 :鉴定雄性胚胎的准确率为 83% ( 1 5 / 1 8)。鉴定雌性胚胎的准确率为 94 % ( 1 5 / 1 6)。
|
| 关 键 词： | 间接ELISA HY单抗 间接免疫荧光法 PCR 小鼠胚胎 |
| 文章编号： | 0258-7033(2001)06-0006-03 |
| 修稿时间： | 2000年5月9日 |
Detection of Monoclonal Antibody Against H-Y Antigen |
| |
| NIU Shu li,LIU Wei min,HANG Su qing,FENG Zi yun.Detection of Monoclonal Antibody Against H-Y Antigen[J].Chinese Journal of Animal Science,2001,37(6):6-8. |
| |
| Authors: | NIU Shu li LIU Wei min HANG Su qing FENG Zi yun |
| |
| Abstract: | Detection methods of monoclonal antibody against H Y antigen were established by optimized faction of indirected ELISA. After BALB/c female mice were immunized by intraperitoncal injections of syngenic male spleen cells, monoclonal antibody against H Y antigen was developed. The preimplantation mouse embryoes were used for sexing with an inderect immunofluorescence assay. Final sex of the embreyos was tested with Polymerase Chain Reaction (PCR) by amplification of Y chromosome specific sequene. The results showed that 83%(15/18) H Y positive embryos was male embryos, 94%(15/16)H Y negative embryos was female. |
| |
| Keywords: | Indirected ELISA H Y monoclonal antibody Inderect immunofluorescence assay PCR Mouse embryoe |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
