| 野生豆瓣菜嫩茎无性系建立的研究 |
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| 引用本文： | 张楠,朱姝婉,袁静,邹翠霞,姜长阳.野生豆瓣菜嫩茎无性系建立的研究[J].天津农业科学,2008,14(1):17-19. |
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| 作者姓名： | 张楠 朱姝婉 袁静 邹翠霞 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁,大连,116029 |
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| 摘 要： | 以野生豆瓣菜的嫩茎为外植体,在附加不同浓度的BA、IAA、NAA和2,4-D的MS培养基上进行离体培养。结果表明:诱导豆瓣菜的嫩茎形成愈伤组织的最佳培养基为MS+BA0.5 mg/L+2,4-D1.5 mg/L;诱导愈伤组织分化的最佳培养基为MS+BA0.5 mg/L;分化不定苗生根的理想培养基是1/2MS+IAA 0.2 mg/L;试管苗移栽和扦插的最佳基质是炉灰渣;地栽试管苗具有较抗寒、抗逆性强的特点。
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| 关 键 词： | 豆瓣菜 愈伤组织 无性系 |
| 文章编号： | 1006-6500（2008）01-0017-04 |
| 收稿时间： | 2007-07-09 |
| 修稿时间： | 2007-11-08 |
Study on Asexual System of Naturtium officinale |
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| ZHANG Nan,ZHU Shu-wan,YUAN Jing,ZOU Cui-xia,JIANG Chang-yang.Study on Asexual System of Naturtium officinale[J].Tianjin Agricultural Sciences,2008,14(1):17-19. |
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| Authors: | ZHANG Nan ZHU Shu-wan YUAN Jing ZOU Cui-xia JIANG Chang-yang |
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| Abstract: | The stem-segment of Naturtium officinale were cultured on MS medium with BA,IAA,NAA and 2,4-D at different levels.The results showed that the optimal callus induction medium of tender stem was MS+BA 0.5 mg/L+2,4-D 1.5 mg/L;And differentiation medium of callus induction was MS+BA 0.5 mg/L;Induction of root was better in medium of 1/2 MS+IAA 0.2 mg/L.Ashes from a stove were a good transplanting medium.The tuber seedling of open field cultivation had the character of cold-tolerance and stress resistance. |
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| Keywords: | Naturtium offtcinale callus clone |
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