| 广叶绣球菌谷胱甘肽S-转移酶多样性及光照下URE2P gst的表达分析 |
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| 引用本文： | 肖冬来,张迪,马璐,杨驰,江晓凌,林衍铨.广叶绣球菌谷胱甘肽S-转移酶多样性及光照下URE2P gst的表达分析[J].食用菌学报,2017(3). |
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| 作者姓名： | 肖冬来 张迪 马璐 杨驰 江晓凌 林衍铨 |
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| 作者单位： | 福建省农业科学院食用菌研究所,福建福州,350014 |
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| 基金项目： | 福建省属公益类科研院所基本科研专项(2015R1020-5)，福建省自然科学基金(2016J01133)，福建省农业科学院科技创新团队PI项目(2016PI-44) |
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| 摘 要： | 利用生物信息学方法分析广叶绣球菌(Sparassis latifolia)基因组谷胱甘肽S-转移酶(glutathione Stransferases,GST)编码基因。结果表明:广叶绣球菌基因组可编码27个gst,均含有GST保守的N端或C端结构。27个GST分属于8个不同的家族,其中URE2P家族GST数量最多(8个),OMEGA家族含6个,GTE家族含4个,C1、GHR、SIGMA和GTT家族各含2个,EF1Bγ家族含1个。各家族间的GST具有相似的保守基序。利用实时荧光定量PCR技术分析了不同光照时长胁迫下6个URE2P家族GST基因的表达动态。结果表明有4个GST基因(MF327518,MF327511,MF327527,MF327514)在诱导48h后表达量最高,2个GST基因(MF327506,MF327510)在诱导96h后表达量最高。
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| 关 键 词： | 广叶绣球菌 谷胱甘肽S-转移酶 光照诱导 实时荧光定量PCR |
Gene Polymorphism of Glutathione S-transferasein Sparassis latifolia and Expression Analysis of URE2P gst under Light Conditions |
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| XIAO Donglai,ZHANG Di,MA Lu,YANG Chi,JIANG Xiaoling,LIN Yanquan.Gene Polymorphism of Glutathione S-transferasein Sparassis latifolia and Expression Analysis of URE2P gst under Light Conditions[J].Acta Edulis Fungi,2017(3). |
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| Authors: | XIAO Donglai ZHANG Di MA Lu YANG Chi JIANG Xiaoling LIN Yanquan |
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| Abstract: | Using bioinformatics tools,gene polymorphism of glutathione S-transferase (GST) in Sparassis latifolia was analyzed.The S.latifolia genome encoded 27 gst,all of which contained GST conserved domains in either N or C terminals.The 27 GSTs were categorized into 8 different families,including URE2P (8 GST),OMEGA (6 GST),GTE (4 GST),C1 (2 GST),GHR (2 GST),SIGMA (2 GST),GTT (2 GST) and EF1Bγ (1 GST).The GSTs in these different families shared similar conserved sequence motifs.Using quantitative real-time fluorescence PCR,expression kinetics of 6 GST genes in URE2P family were analyzed under different light conditions.Among all tested conditions,four gst (MF327518,MF327511,MF327527,MF327514) showed the highest expression level under 48 h light induction whereas two gst (MF327506,MF327510) showed the highest expression level under 96 h light induction. |
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| Keywords: | Sparassis latifolia glutathione S-transferase light induction quantitative real-time fluorescence PCR |
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