两株西藏白肉灵芝菌株特性初探 |
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引用本文: | 胡惠萍,刘远超,莫伟鹏,黄龙花,张一帆,李泰辉,陈蓉,谢意珍.两株西藏白肉灵芝菌株特性初探[J].食用菌学报,2017(1):50-54. |
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作者姓名: | 胡惠萍 刘远超 莫伟鹏 黄龙花 张一帆 李泰辉 陈蓉 谢意珍 |
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作者单位: | 1. 广东省微生物研究所,省部共建华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东广州 510070;2. 广东粤微食用菌技术有限公司,广东广州,510663;3. 林芝市科技开发交流服务中心,西藏林芝,860000;4. 广东省微生物研究所,省部共建华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东广州 510070;广东粤微食用菌技术有限公司,广东广州510663 |
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基金项目: | 广东省科技计划项目(2016B020212008),广州市科技计划项目(201604020005) |
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摘 要: | 对西藏林芝地区的白肉灵芝(Ganoderma leucocontextum)菌株G-122和G-241进行鉴别分析,并对其栽培性状、有效成分含量和提取物功能等方面进行研究。基于形态学特性和ITS分析,2个菌株均鉴定为白肉灵芝。二者菌丝对峙培养产生明显拮抗线,菌丝体生物量差异显著(G-241高于G-122),子实体颜色不同(G-241和G-122分别为红褐色和黄褐色),G-241子实体单菇重高于G-122;有效成分含量差异也较大,G-122粗多糖含量高而G-241总三萜含量高;菌株G-122、G-241醇提物对乳腺癌MT-1细胞IC_(50)值分别为265.2和225.6μg/mL。实验结果表明菌株G-122和G-241为不同菌株。
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关 键 词: | 白肉灵芝 三萜 抗肿瘤 多糖 |
Isolation,Characterization and Anti-cancer Activityof Two Ganoderma leucocontextum Strains |
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Abstract: | Two Ganoderma isolates were collected from the wild near Linzhi City, Tibet, and identified as two separate strains of G.leucocontextum (G-122 and G-241) based on mycelial growth antagonism, fruit body morphology and ITS sequence analysis.Fruit body color also differed (G-122 produced yellow-brown pilei compared to the red-brown pilei of G-241), and the average weight of individual fruit bodies and the biological efficiency were both higher in the case of G-241.Ethanolic extracts of G-122 contained higher levels of crude polysaccharide compared to triterpenoids, whereas the reverse was true for G-241.Both G-122 and G-241 extracts inhibited the proliferation of MT-1 breast cancer cells with IC50 values of 265.2 and 225.6 μg/mL, respectively. |
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Keywords: | Ganoderma leucocontextum triterpenoid anti-neoplastic polysaccharide |
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