| 无籽刺梨的组织培养研究 |
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| 引用本文： | 李斌,林源,唐军荣,李永和,尹丽莎,辛培尧.无籽刺梨的组织培养研究[J].经济林研究,2016(3):142-147. |
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| 作者姓名： | 李斌 林源 唐军荣 李永和 尹丽莎 辛培尧 |
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| 作者单位： | 1. 西南林业大学 云南 昆明 650224;2. 大理农林职业技术学院,云南 大理,671003 |
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| 基金项目： | 云南省林学一级学科博士点建设项目；云南省林业厅省级林业科技推广项目(〔2014〕TSYN25)；国家自然科学基金项目(31360184)。 |
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| 摘 要： | 为了建立无籽刺梨组织培养技术体系,从而有效解决生产用苗紧缺问题,以无籽刺梨带叶腋的嫩茎为外植体进行了组织培养试验,研究了不同消毒时长、培养基种类及激素浓度对无籽刺梨外植体消毒效果、分化培养、增殖培养及生根培养的影响情况。结果表明:外植体经清水冲洗后用75%的酒精消毒20 s,以0.1%的升汞消毒9 min,污染率低至0;培养基1/2MS+0.50 mg/L TDZ+0.02 mg/L 2,4-D+5.0 mg/L Ag NO_3适用于无籽刺梨叶柄的分化培养,分化率为50.0%;适用于无籽刺梨增殖的培养基为MS+0.50 mg/L 6-BA+0.10 mg/L NAA,增殖倍数达5.56;在1/2MS+0.10 mg/L IBA+0.20 mg/L NAA+0.30 g/L活性炭的培养基上,无籽刺梨的生根率为92.5%;在腐殖土︰红土︰珍珠岩=1︰1︰1的基质中炼苗,无籽刺梨组培苗的成活率可达97.2%。
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| 关 键 词： | 无籽刺梨 组织培养 培养基 |
Tissue culture techniques in Rosa sterilis |
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| Abstract: | In order to establish the system of tissue culture techniques in Rosa sterilis, and to solve the problem of insufficient supply of R. sterilis seedlings in production, using tender stems in R. sterilis with leaf axils as explants, the effects of disinfection time, medium types and hormone concentrations on disinfection effect, differentiation culture, proliferation culture and rooting culture were studied. The results showed that after rinsed with water, the explants were disinfected with 75% alcohol for 20 s and 0.1% mercuric chloride for 9 min, the contamination rate was 0. 1/2MS + 0.50 mg/L TDZ + 0.02 mg/L 2,4-D + 5 mg/L AgNO3 was suitable for differentiation culture of R. sterilis petioles, and the differentiation rate was 50.0%. MS + 0.50 mg/L 6-BA + 0.10 mg/L NAA was suitable for R. sterilis proliferation culture, and the proliferation multiple was 5.56. In the culture medium of 1/2MS + 0.10 mg/L IBA + 0.20 mg/L NAA + 0.30 g/L activated carbon, the rooting rate was 92.5%. In the mixed matrix of humus, laterite and perlite (1 ︰ 1 ︰ 1), the survival rate of tissue culture seedlings in R. sterilis was 97.2%. |
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| Keywords: | Rosa sterilis tissue culture medium |
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