| 禽副粘病毒2型Yucaipa标准株F基因的克隆和序列分析 |
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| 引用本文： | 卜春亚,赵继勋,杨爱梅.禽副粘病毒2型Yucaipa标准株F基因的克隆和序列分析[J].中国预防兽医学报,2003,25(4):254-257. |
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| 作者姓名： | 卜春亚 赵继勋 杨爱梅 |
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| 作者单位： | 中国农业大学,实验动物研究所,北京,100094 |
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| 摘 要： | 根据从GenBank上查到的PMV-2型Yucaipa株F基因序列——D13977序列，设计特异性引物，从禽副粘病毒2型Yueaipa标准株成功地克隆了F基因全编码序列。所测的核苷酸序列大小为1654bp，编码一个含536个氨基酸残基的多肽(未加工前)，分子量约为55．75kDa。裂解位点在F基因的第98和第99个氨基酸残基之间，F2蛋白羧基端裂解位点区的氨基酸残基序列为Lys-Pro-Ala-Ser-Arg。F基因序列同源性比较结果表明本研究所测的序列是PMV-2型F基因序列，并进一步证实了副粘病毒2型与分离自猴的Murayama病毒在系统进化上有非常紧密的关系。
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| 关 键 词： | 副粘病毒2型 F基因 RT－PER 序列分析 |
| 文章编号： | 1008-0589(2003)04-0254-04 |
| 修稿时间： | 2002年10月16 |
Molecular cloning and sequence analysis of the fusion glycoprotein of avian paramyxovirus type 2 viruses-strain Yucaipa |
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| BU Chun_ya,ZHAO Ji_xun,YANG Ai_mei.Molecular cloning and sequence analysis of the fusion glycoprotein of avian paramyxovirus type 2 viruses-strain Yucaipa[J].Chinese Journal of Preventive Veterinary Medicine,2003,25(4):254-257. |
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| Authors: | BU Chun_ya ZHAO Ji_xun YANG Ai_mei |
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| Abstract: | According to GenBank D13977 sequence, F gene of PMV_2 strain Yucaipa was successfully cloned and sequenced. The sequenced F gene is 1 654 nucleotide long and contain a single open reading frame encoding a polypeptide of 536 amino acids with a predicted molecular weight (MW) of 55.75 kDa. F0 protein is cut into F2 and F1 protein between the 98th and 99th amino acid residue. The F2/F1 cleavage site in carboxyl terminus of F2 protein is Lys_Pro_ Ala_ Ser_ Arg, which is similar to that of Murayama virus (Lys_Pro_Thr _ Ala _Arg). Comparative analysis of F gene sequences of PMV_2 viruses showed that the sequence was PMV_2 F gene sequence. Murayama virus isolated from Cynomologus Monkeys was also proved to have close relationship with paramyxovirus type 2 by comparing F genes of PMV_2 with that of Murayama virus. |
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| Keywords: | paramyxovius type 2 F gene RT_PCR sequence analysis |
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