| 1-磷酸甘露醇脱氢酶基因转化水稻的研究 |
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| 引用本文： | 王慧中,刘俊君,卢德赵,赵艳,颜美仙,钱前,彭学贤,陈受宜,黄大年.1-磷酸甘露醇脱氢酶基因转化水稻的研究[J].中国水稻科学,2003,17(1):6-10. |
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| 作者姓名： | 王慧中 刘俊君 卢德赵 赵艳 颜美仙 钱前 彭学贤 陈受宜 黄大年 |
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| 作者单位： | 1.杭州师范学院 生物化学与分子生物学重点实验室， 浙江 杭州 310036;2.中国水稻研究所， 浙江 杭州 310006； 3中国科学院 遗传与发育生物学研究所 植物生物技术开放实验室， 北京 100101;4.杭州商学院, 浙江 杭州 310035 |
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| 基金项目： | 浙江省自然科学基金资助项目(3982 97，30 2 0 61 )，国家自然科学基金资助项目(39970 4 0 9)，中国水稻科学发展基金资助项目 (0 0 0 32 39) |
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| 摘 要： | 摘要：PCR和Southern blotting检测表明，来自大肠杆菌的i]mtlD/i]基因已通过农杆菌介导整合进水稻基因组。i]mtlD/i]基因在T1代出现分离, T2代出现纯系。在0.75% NaCl胁迫下，7个转基因T3代株系都能检测到mtlD酶活性，与对照相比细胞膜的相对电导率和大分子渗漏值明显降低。部分转基因株系能在 1.0% NaCl浓度下正常生长，而对照在0.5% NaCl浓度下已不能存活。通过有性杂交途径实现了i]mtlD/i]和i]gutD/i]两个基因的聚合，部分杂交后代株系能在1.25%NaCl胁迫下正常生长结实。
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| 关 键 词： | 水稻 1-磷酸甘露醇脱氢酶基因 6-磷酸山梨醇脱氢酶基因 转基因植株 耐盐性 有性杂交 |
| 文章编号： | 1001-7216(2003)01-0006-05 |
| 收稿时间： | 1900-01-01; |
Transformation of Rice with mtlD Gene |
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| WANG Hui zhong ,LIU Jun jun ,LU De zhao ,ZHAO Yan ,YAN Mei xian ,QIAN Qian ,PENG Xue xian ,CHEN Shou yi ,HUANG Da nian.Transformation of Rice with mtlD Gene[J].Chinese Journal of Rice Science,2003,17(1):6-10. |
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| Authors: | WANG Hui zhong LIU Jun jun LU De zhao ZHAO Yan YAN Mei xian QIAN Qian PENG Xue xian CHEN Shou yi HUANG Da nian |
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| Institution: | （;1. Key Laboratory of Biochemistry and Molecular Biology; Hangzhou Normal College; Hangzhou 310036; China;2.China National Rice Research Institute; Hangzhou 310006;3.Laboratory of Plant Biotechnology; Institute of Genetics and Developmental Biology; Chinese Academy of Sciences; Bejing 100101;4.Hangzhou Institute of Commerce, Hangzhou 310035, China) |
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| Abstract: | PCR and Southern blot analysis indicated that mtlD gene had been integrated into the rice genome mediated by Agrobacterium tumefaciens LBA4404 (pBIM). mtlD gene segragated in T1 generation and pure transgenic lines appeared in T2 generation. The expression of the mtlD gene in transgenic plants was demonstrated by enzymatic activity assay. Under 0.75% NaCl stress, the relative electronic conductivity and membrane permeability were detected in T3 transgenic plants of 7 different lines. Results showed that the damage to membrane structure of the transgenic plant was lower than that of their controls. Some transgenic plant lines could grow normally under 1.0% NaCl stress whereas the controls could not grow and died after 2 weeks under the same environment. Through sexual hybridization, rice plants with mtlD gene and gutD gene were got, some of them could grow normally under the stress of 1.25% NaCl. |
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| Keywords: | rice mtlDgene gutD gene transgenic plant salt tolerance sexual hybridization |
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