| 圆叶柳无性系建立的研究 |
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| 引用本文： | 宁婵,贾双,孙萌,徐娜,姜长阳.圆叶柳无性系建立的研究[J].吉林林业科技,2011,40(3):1-4. |
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| 作者姓名： | 宁婵 贾双 孙萌 徐娜 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁,大连,116029 |
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| 基金项目： | 辽宁省高等教育教学改革资助项目，辽宁师范大学教学改革资助项目 |
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| 摘 要： | 以圆叶柳即将萌动的生长点为材料,进行生长点生长与分化、试管苗生根、移栽和移植的研究,建立起圆叶柳无性系.结果表明:生长点生长培养的适宜培养基为MS+6-BA0.1 mg·L-1+GA30.5 mg·L-1+NAA0.2 mg·L-1;生长芽分化培养的适宜培养基为MS+NAA0.1 mg·L-1+6-BA0.5 mg·L...
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| 关 键 词： | 圆叶柳 愈伤组织 无性系 |
Establishment of clone of Salix rotundifolia |
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| NING Chan,JIA Shuang,SUN Meng,XU Na,JIANG Chang-Yang.Establishment of clone of Salix rotundifolia[J].Jilin Forestry Scicnce and Technology,2011,40(3):1-4. |
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| Authors: | NING Chan JIA Shuang SUN Meng XU Na JIANG Chang-Yang |
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| Institution: | (College of Life Science,Liaoning Normal University,Dalian 116029,China) |
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| Abstract: | The sprouts of Salix rotundifolia were taken to establish the clone of S. rotundifolia by growing point growth and differentiation, tube seedlings rooting, and transplantation. The results show that MS ＋ BA0.1 mg·L^- ＋ GA30.5 mg ·L^- ＋ NAA0.2 mg ·L^- is the ideal medium for growing point culture, MS ＋ NAAO. 1 m g ·L^- ＋6 - BA0.5 mg·L^- and 1.0 mg·L^-＇ is suitable medium for differentiation of growing buds, and 1/ 2MS ＋ IAA0. 1 mg ·L^- ＋ NAA0.2 - 0.4 mg ·L^- is the best medium for roofing and subcultures of differentiated buds. The survival rate of the tube seedlings grown on substrate with river sand was 90.4%. Tube seed- lings were transplanted to sidehill understory. The transplanted seedlings grow strong, with 98.3% survival rate, and keep all biological characteristics of Salix rotundifolia. |
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| Keywords: | Salix rotundifolia callus clone |
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