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| 不同猪种TGF-β1基因单核苷酸多态性分析 | |
| 引用本文： | 武艳萍,傅金恋,李宁,王晓凤,卢纪和,王爱国.不同猪种TGF-β1基因单核苷酸多态性分析[J].中国畜牧杂志,2005,41(4):11-13. |
| 作者姓名： | 武艳萍 傅金恋 李宁 王晓凤 卢纪和 王爱国 |
| 作者单位： | 1. 中国农业大学动物科技学院,农业部畜禽遗传育种重点开放实验室,北京,100094 2. 中国农业大学农业生物技术国家重点实验室,北京,100094 3. 北京养猪育种中心,北京,100085 |
| 基金项目： | 国家高技术研究发展计划项目(No.2002AA242031)，北京市科技计划项目 (No.H022020050091) |
| 摘 要： | 以二花脸猪、大白猪、长白猪、皮特兰猪和圣特西猪共计861 头为研究材料,采用PCR-SSCP技术,对猪TGF β1基因6 7外显子区的1156 bp序列进行多态性分析,发现一个多态位点。经克隆测序分析,位于第6内含子区内存在C→T突变,该突变位点为第7外显子上游的第9位碱基(序列:AJ621785中的第1043位点)。对不同猪群的基因型和基因频率统计结果表明,二花脸猪以等位基因T为主,而大白猪、长白猪、皮特兰猪和圣特西猪则以等位基因C为主,且各猪群均处于Hardy Weinberg平衡。 |
| 关 键 词： | 畜牧、兽医科学基础学科 TGFβ1 PCRSSCP SNP 猪 |
| 文章编号： | 0258-7033(2005)04-0011-03 |
| 修稿时间： | 2004年12月28 |
Analysis on Single Nucleotide Polymorphism of Porcine Transforming Growth Factor beta 1 Gene in Different Pig Breeds |
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| WU Yan-ping,FU Jin-lian,LI Ning,WANG Xiao-feng,LU Ji-he,WANG Ai-guo.Analysis on Single Nucleotide Polymorphism of Porcine Transforming Growth Factor beta 1 Gene in Different Pig Breeds[J].Chinese Journal of Animal Science,2005,41(4):11-13. | |
| Authors: | WU Yan-ping FU Jin-lian LI Ning WANG Xiao-feng LU Ji-he WANG Ai-guo |
| Abstract: | By PCR-SSCP approach, a single nucleotide polymorphism (SNP) of porcine transforming growth factor beta1(TGF-β1) was identified in different breeds including Large White, Landrace, Pietrain, Synthethic and Erhualian. Comparative sequencing of cloning product revealed a C→T transition in intron 6 at position 1043 of partial TGF-β1 gene, exons 6-7 (AJ621785). For the SNP, 861 individuals were investigated. The allele C dominates in the lean-type pig breeds such as Large White, Landrace, Pietrain, Synthethic, however, in Erhualian, the allele T was in majority. All the population were in Hardy-Weinberg equilibrium state. |
| Keywords: | basal disciplines of animal and veterinary science TGF-β1 PCR-SSCP single nucleotide polymorphism (SNP) pig |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |