| 利用24个微卫星进行猪数量性状座位定位及其遗传效应分析 |
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| 引用本文： | 左波,熊远著,苏玉虹,邓昌彦,郑嵘,雷明刚,余雳.利用24个微卫星进行猪数量性状座位定位及其遗传效应分析[J].畜牧兽医学报,2003,34(2):139-146. |
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| 作者姓名： | 左波 熊远著 苏玉虹 邓昌彦 郑嵘 雷明刚 余雳 |
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| 作者单位： | 1. 华中农业大学农业部猪遗传育种重点开放实验室,武汉,430070
2. 锦州医学院基础学院,锦州,121001 |
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| 基金项目： | “973”国家重点基础研究发展规划项目 (G2 0 0 0 0 16 10 5 )资助 |
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| 摘 要： | 以 3头英系大白公猪与 7头梅山母猪杂交产生的三代资源家系用来检测猪重要经济性状的数量性状座位(QTL) ,2 0 0 0年下半年随机选留 140头F2代个体 ,进行屠宰测定 ,记录了包括生长、胴体组成等 43个性状 ;从已定位于家猪 3、4和 7号染色体上的遗传标记中选用 2 4个微卫星标记对所有个体进行基因型检测。采用最小二乘回归区间定位法进行QTL检测 ,通过置换实验来确定显著性阈值。在所研究的 32个生长和胴体性状中 ,3条染色体总共 16个QTL达到染色体显著水平 (P <0 0 5 ) ,其中 4个达到染色体极显著性水平 (P <0 0 1) ;同时在 4号和 7号染色体上还检测到了影响器官重性状的 3个QTL ,达到了染色体显著水平 (P <0 0 5 )。在某些QTL座位 ,其有利等位基因来源于具有较低性状平均值的品种。 2QTL模型分析下 ,在 4号染色体上检测到影响板油重的 2个QTL ,并且它们的效应方向相反。
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| 关 键 词： | 猪 微卫星 数量性状座位定位 遗传效应 |
| 文章编号： | 0366-6964(2003)02-0139-08 |
Mapping and Genetic Effect Analysis of Swine Quantitative Trait Loci Using 24 Microsatellites |
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| ZUO Bo,XIONG Yuan-zhu,SU Yu-hong,DENG Chang-yan,ZHENG Rong,LEI Ming-gang,YU Li.Mapping and Genetic Effect Analysis of Swine Quantitative Trait Loci Using 24 Microsatellites[J].Acta Veterinaria et Zootechnica Sinica,2003,34(2):139-146. |
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| Authors: | ZUO Bo XIONG Yuan-zhu SU Yu-hong DENG Chang-yan ZHENG Rong LEI Ming-gang YU Li |
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| Institution: | ZUO Bo1,XIONG Yuan-zhu1,SU Yu-hong2,DENG Chang-yan1,ZHENG Rong1,LEI Ming-gang1,YU Li1 |
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| Abstract: | A three-generation resource family was established using 3 English Large-White sires and 7 Mei-Shan dams to detect quantitative trait loci(QTL) that control quantitative traits of economic importance.140 F2 individuals were randomly selected at the last half-year of 2000,and were slaughtered for testing.43 traits including growth performance and carcass composition were recorded.Animals were genotyped for 24 microsatellites on SSC3 (Sus scrofachromosome 3),SSC4 and SSC7.Least square regression interval mapping was used for QTL detection,and significance thresholds were determined by permutation tests.For carcass composition and growth traits,a total of 16 QTL were significant at the 5% chromosome-wise level on these chromosomes,of which 4 were significant at the 1% chromosome-wise level.Meanwhile,3 significant QTL affecting organ weight were detected at the 5% chromosome-wise level on SSC4 and SSC7.For some QTL the favorable allele originated from the breed with the lower trait mean.Furthermore,under two QTL model analysis,there exist 2 QTL influencing leaf fat weight(LFW) on SSC4,and their effects were opposite. |
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| Keywords: | Swine Microsatellites Quantitative trait loci(QTL)mapping Genetic effect |
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