| 酶解法提取地鳖虫多糖工艺的优化 |
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| 引用本文： | 罗志文,刘娟,赵永勋.酶解法提取地鳖虫多糖工艺的优化[J].黑龙江农业科学,2012(8):102-104. |
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| 作者姓名： | 罗志文 刘娟 赵永勋 |
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| 作者单位： | 1. 佳木斯大学生命科学学院,黑龙江佳木斯154007
2. 佳木斯大学药学院,黑龙江佳木斯154007 |
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| 基金项目： | 黑龙江省教育厅科学技术资助项目(11531384) |
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| 摘 要： | 采用酶解法对地鳖虫多糖进行提取试验,优化提取工艺.通过单因素和正交试验方法,筛选提取条件,测定多糖含量,摸索地鳖虫多糖提取的最佳酶用量、提取时间和温度.结果表明:地鳖虫多糖提取的最佳酶用量为300μg?g‐1,提取温度50℃,提取时间3 h ,多糖含量为14.326%.
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| 关 键 词： | 地鳖虫多糖 酶解法 提取工艺 提取条件优化 |
Enzymatic Extraction of Eupolyphaga sinensis Polysaccharide Technology Optimization |
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| LUO Zhi-wen,LIU Juan,ZHAO Yong-xun.Enzymatic Extraction of Eupolyphaga sinensis Polysaccharide Technology Optimization[J].Heilongjiang Agricultural Science,2012(8):102-104. |
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| Authors: | LUO Zhi-wen LIU Juan ZHAO Yong-xun |
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| Institution: | 1 (1.Life Sciences College of Jiamusi University,Jiamusi,Heilongjiang 154007;2.Medicine College of Jiamusi University,Jiamusi,Heilongjiang 154007) |
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| Abstract: | The Eupolyphaga sinensis polysaccharides extraction test was conducted and extraction process was optimized by enzymatic hydrolysis.The extraction condition was selected and the content of polysaccharide was determined through the single factor and orthogonal test method,and the optimum enzyme dosage,extraction time and temperature of Eupolyphaga sinensis polysaccharide were studied.The results showed that the optimum conditions of Eupolyphaga sinensis polysaccharide was extraction enzyme dosage of 300 μg·g-1,extraction temperature of 50℃,extraction time 3 h,the content of polysaccharides was 14.326%. |
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| Keywords: | Eupolyphaga sinensis polysaccharide enzymatic hydrolysis extraction extraction condition optimization |
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