| EMS诱变筛选马铃薯茎段离体耐盐变异体 |
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| 引用本文： | 杨乾,张峰,王蒂,张俊莲,杨宏羽,刘玉汇.EMS诱变筛选马铃薯茎段离体耐盐变异体[J].核农学报,2011,25(4):673-678. |
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| 作者姓名： | 杨乾 张峰 王蒂 张俊莲 杨宏羽 刘玉汇 |
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| 作者单位： | 1. 甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070／甘肃农业大学农学院,甘肃兰州730070
2. 甘肃农业大学农学院,甘肃兰州,730070 |
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| 基金项目： | 甘肃省马铃薯新品种选育扶持项目，甘肃省农业生物技术研究与应用开发项目 |
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| 摘 要： | 用甲基磺酸乙酯（EMS）诱变处理甘农薯2号和青薯2号试管苗茎段,进行耐盐性研究。结果表明,甘农薯2号和青薯2号试管苗离体茎段诱变处理的适宜浓度和时间组合为0.9%～1.0%＋4.0h。再通过1.0%NaCl选择压进行耐盐性变异体筛选,获得了耐1.0%NaCl诱变植株。在盐胁迫条件下诱变株超氧化物歧化酶（SOD）活性增强...
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| 关 键 词： | 马铃薯 甲基磺酸乙酯(EMS) 离体筛选 耐盐性 变异体 |
| 收稿时间： | 2011-01-20 |
SELECTION OF SALT-TOLERANT VARIANTS FROM POTATO in vitro MICRO-CUTTINGS INDUCED BY EMS |
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| YANG Qian,ZHANG Feng,WANG Di,ZHANG Jun-lian,YANG Hong-yu,LIU Yu-hui.SELECTION OF SALT-TOLERANT VARIANTS FROM POTATO in vitro MICRO-CUTTINGS INDUCED BY EMS[J].Acta Agriculturae Nucleatae Sinica,2011,25(4):673-678. |
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| Authors: | YANG Qian ZHANG Feng WANG Di ZHANG Jun-lian YANG Hong-yu LIU Yu-hui |
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| Institution: | 1. Gansu Key Laboratory of Crop Genetic &; Germplasm Enhancement, Lanzhou, Gansu 730070;
2. College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070 |
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| Abstract: | In vitro micro-cuttings of potato cv. Gannongshu 2 and cv.Qingshu 2 were used as materials to study induction effect of ethyl methyl sulfonate (EMS) on selection of salt-tolerance variants. The results showed that the optimun, EMS concentration was 0.9%~1.0% and the most suitable treated duration was 4 hours for both micro-cuttings. The materials were treated by EMS (0.9%~1.0%+4.0h) and then salt-tolerance variants under 1.0% NaCl stress was selected. The variants presented stronger superoxide dismutase (SOD) activity, less cell membrane permeability, higher content of free proline and chlorophyll than the control, which indicated that the variants have stronger salt-tolerance. |
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| Keywords: | potato ethyl methyl sulfonate（EMS） in vitro screening salt tolerance variant |
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