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| OsCRY2基因抑制表达对水稻主要农艺性状的影响 | |
| 引用本文： | 李毓,庄春红,庄伟建,王乃元,戴飞,仇秀丽,谢娜英,林剑钦.OsCRY2基因抑制表达对水稻主要农艺性状的影响[J].核农学报,2011,25(6):1094-1099. |
| 作者姓名： | 李毓 庄春红 庄伟建 王乃元 戴飞 仇秀丽 谢娜英 林剑钦 |
| 作者单位： | 1. 福建农林大学作物科学学院,福建福州350002／福建农林大学作物遗传育种与综合利用教育部重点实验室,福建福州350002 2. 福建农林大学作物科学学院,福建福州350002／福建省分子与细胞生物学重点实验室,福建福州350002 3. 福建农林大学作物遗传育种与综合利用教育部重点实验室,福建福州,350002 4. 福建省分子与细胞生物学重点实验室,福建福州,350002 5. 福建农林大学作物科学学院,福建福州,350002 |
| 基金项目： | 福建省科技计划重点项目，国家高技术研究发展计划("863计划")项目 |
| 摘 要： | 利用已公布的水稻OsCRY2基因序列设计引物,扩增部分基因片段,成功构建了ihpRNA植物表达载体pSC1301-347-OsCRY2,并通过农杆菌介导法将OsCRY2干扰片段导入水稻获得了转基因植株。根据转基因植株主要农艺性状的表现,分析了该基因的功能。结果表明,抑制OsCRY2基因表达会强烈延迟水稻开花与成熟;转基... |
| 关 键 词： | 水稻 OsCRY2基因 ihpRNA表达载体 转化 |
| 收稿时间： | 2011-04-22 |
| 修稿时间： | 2011-09-13 |
EFFECTS OF SUPPRESSING OsCRY2 GENE EXPRESSION ON MAIN AGRONOMIC TRAITS OF RICE |
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| LI Yu,ZHUANG Chun-hong,ZHUANG Wei-jian,WANG Nai-yuan,DAI Fei,QIU Xiu-li,XIE Na-ying,LIN Jian-qin.EFFECTS OF SUPPRESSING OsCRY2 GENE EXPRESSION ON MAIN AGRONOMIC TRAITS OF RICE[J].Acta Agriculturae Nucleatae Sinica,2011,25(6):1094-1099. | |
| Authors: | LI Yu ZHUANG Chun-hong ZHUANG Wei-jian WANG Nai-yuan DAI Fei QIU Xiu-li XIE Na-ying LIN Jian-qin |
| Institution: | 1. College of Crop Science, Fujian Agriculture and Forestry University,Fuzhou, Fujian 350002; 2. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University,Fuzhou, Fujian 350002; 3. Key Laboratory of Molecular and Cellular Biology, Fujian Province,Fuzhou, Fujian 350002 |
| Abstract: | According to the published rice OsCRY2 gene sequence, we designed the related primers to obtain part of the gene byPCRandconstructed an ihpRNA plant expression vector named pSC1301-347-OsCRY2. The OsCRY2 gene interference fragment was then introduced into rice by Agrobacterium-mediated transformation. The functions of the gene was preliminarily analyzed by the performance of the transformants. The results indicated that the transgenic plants in which the expression of OsCRY2 gene was suppressed give rise to both retarded flowering and maturing. Moreover , the transgenic plants have short panicles and flag leaves, lower seed-set percent with the grain becoming longer while the other agronomic traits do not change apparently. |
| Keywords: | rice OsCRY2 gene ihpRNA expression vector transformation |
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