| 分光光度法测定俄罗斯鲟精子密度标准的研究 |
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| 引用本文： | 张涛,章龙珍,庄平,田美平,刘鉴毅,赵峰,高露姣,冯广朋,黄晓荣,闫文罡.分光光度法测定俄罗斯鲟精子密度标准的研究[J].海洋渔业,2009,31(1). |
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| 作者姓名： | 张涛 章龙珍 庄平 田美平 刘鉴毅 赵峰 高露姣 冯广朋 黄晓荣 闫文罡 |
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| 作者单位： | 1. 中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海,200090
2. 上海海洋大学生命科学与技术学院,上海,201306 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金资助项目(中国水产科学研究院东海水产研究所)资助项目，国家高技术研究发展计划(863计划)，农业科技成果转化基金，浙江省重大科技专项项目，上海市教育委员会E-研究院建设计划 |
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| 摘 要： | 为了标准化精子超低温冷冻保存和人工授精程序,建立了分光光度法测定俄罗斯鲟精子密度的方法,比较了不同波长(380 nm、530 nm、780 nm)下吸光度(A)与精子密度(C)的关系。结果表明,分光光度法的检测下限为3×106cells/mL,且检测上限随波长的增加而上升,当精子密度为3×106~1.5×109cells/mL时,530 nm为最适检测波长,吸光度与精子密度呈对数回归关系,其回归方程为:A530=-8.560+1.323 lgC(R2=0.971)。
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| 关 键 词： | 俄罗斯鲟 精子密度 分光光度法 |
Determination on the sperm concentration standard of Russian sturgeon (Acipenser gueldenstaedtii) by means of spectrophotometric method |
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| ZHANG Tao,ZHANG Long-zhen,ZHUANG Ping,TIAN Mei-ping,LIU Jian-yi,ZHAO Feng,GAO Lu-jiao,FENG Guang-peng,HUANG Xiao-rong,YAN Wen-gang.Determination on the sperm concentration standard of Russian sturgeon (Acipenser gueldenstaedtii) by means of spectrophotometric method[J].Marine Fisheries,2009,31(1). |
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| Authors: | ZHANG Tao ZHANG Long-zhen ZHUANG Ping TIAN Mei-ping LIU Jian-yi ZHAO Feng GAO Lu-jiao FENG Guang-peng HUANG Xiao-rong YAN Wen-gang |
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| Abstract: | To provide the necessary standardization for procedures of sperm cryopreservation and artificial insemination,a spectrophotomeric method has been established to determine the sperm concentration of Russian sturgeon,Acipenser gueldenstaedtii.Wavelengths of 380,530,and 780 nm have been compared,and 530 nm was found to be the most sensitive and reliable.A logarithmic relationship between sperm concentration(C) and photometric absorbance(A) was observed for sperm concentration between 3 × 106 and 1.5 × 109 cells/mL.The regression equation for the standard curve at 530 nm was A530=-8.560+1.323 lgC(R2 = 0.971). |
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| Keywords: | Acipenser gueldenstaedtii sperm concentration spectrophotomeric method |
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