| GFP标记的尖孢镰刀菌西瓜专化型侵染西瓜过程观察 |
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| 引用本文： | 李春强,梁慧施,夏亦荠,彭明.GFP标记的尖孢镰刀菌西瓜专化型侵染西瓜过程观察[J].热带作物学报,2011,32(10):1935-1939. |
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| 作者姓名： | 李春强 梁慧施 夏亦荠 彭明 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,海南海口 571101;农业部热带作物生物技术重点实验室,海南海口 571101
2. 香港浸会大学生物系,中国香港 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费(No.ITBBZX0821); 公益性行业(农业)科研专项(No.200903049); 海南省自然基金项目(No.310078) |
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| 摘 要： | 将绿色荧光蛋白基因转入西瓜尖孢镰刀菌FON中,并利用荧光共聚焦显微镜观察GFP标记菌株侵染西瓜的过程.结果显示,转化子连续转接4代能够稳定遗传,荧光强度良好,PCR验证gfp基因已转入菌株FON中；利用GFP标记菌株在荧光共聚焦显微镜下观察其侵染西瓜苗的过程,发现在1/2 MS培养的西瓜苗中,FON经过48 h侵染即可进入西瓜的根维管束,第3天便进入茎维管束,第4天进入叶维管束(包括叶柄和叶脉)；在土壤盆栽条件下,侵染后2d FON进入西瓜的根维管束,第9天进入茎维管束,第11天进入叶维管束.培养基培养与土壤盆栽相比,培养基栽培FON侵染的速度更快.
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| 关 键 词： | 尖孢镰刀菌西瓜专化型 GFP 西瓜 侵染过程 |
Observation of the Infection Process of Watermelon by Fusarium oxysporum f. sp. niveum Using the GFP Marker |
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| LI Chunqiang,LIANG Huishi,XIA Yiji and PENG Ming.Observation of the Infection Process of Watermelon by Fusarium oxysporum f. sp. niveum Using the GFP Marker[J].Chinese Journal of Tropical Crops,2011,32(10):1935-1939. |
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| Authors: | LI Chunqiang LIANG Huishi XIA Yiji and PENG Ming |
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| Institution: | LI Chunqiang1,2,LIANG Huishi3,XIA Yiji3,PENG Ming1,2 1 Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China 2 Key Laboratory of Tropical Corp Biotechnology,Ministry of Agriculture,China 3 Department of Biology Faculty of Science,Hong Kong Baptist University,Hong Kong,China |
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| Abstract: | Fusarium oxysporum f.sp.Niveum(FON)was successfully transformed with the gene coding for green fluorescent protein(GFP).Transformants could emit quite stable fluorescence under 488 nm laser after four successive subcultures.PCR analysis confirmed that the gfp gene was transformed into FON.Observation of watermelon infection process by the GFP expressing FON strain was then carried out.Watermelon grown on 1/2 MS medium and soil were used for the study and their roots were inoculated with the FON strain.Resul... |
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| Keywords: | Fusarium oxysporum f sp niveum Green fluorescent protein Watermelon Infection process |
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