| 利用微卫星多态性揭示酿酒酵母菌株遗传多样性 |
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| 引用本文： | 冯敏,王春晓,刘延琳.利用微卫星多态性揭示酿酒酵母菌株遗传多样性[J].中国农业科学,2012,45(12):2537-2543. |
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| 作者姓名： | 冯敏 王春晓 刘延琳 |
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| 作者单位： | 西北农林科技大学葡萄酒学院，陕西杨凌 712100 |
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| 基金项目： | 国家葡萄产业技术体系建设专项经费资助 |
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| 摘 要： | 【目的】利用微卫星标记技术对来自新疆和宁夏的48株酿酒酵母菌株进行区分，并分析其亲缘关系。【方法】选择4个微卫星位点：SCAAT1、SCYOR267c、C11和YPL009c，用其多态性对48个菌株进行分析。【结果】4个微卫星标记在48株菌中共检测到87个等位基因，39种基因型；4个位点多态信息含量为0.8593-0.9115，均为高度多态位点；期望杂合度（heterozygosity expected, He）和观测杂合度（heterozygosity observed, Ho）分别为0.8803-0.9268和0.8333-0.9792。【结论】宁夏和新疆地区酿酒酵母菌株生物多样性丰富。
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| 关 键 词： | 酿酒酵母 微卫星标记 菌株区分 生物多样性 |
| 收稿时间： | 2011-01-19 |
Genetic Diversity of Saccharomyces cerevisiae Strains Revealed by Microsatellite Sequence Polymorphism |
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| FENG Min
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WANG Chun-xiao
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LIU Yan-lin.Genetic Diversity of Saccharomyces cerevisiae Strains Revealed by Microsatellite Sequence Polymorphism[J].Scientia Agricultura Sinica,2012,45(12):2537-2543. |
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| Authors: | FENG Min WANG Chun-xiao LIU Yan-lin |
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| Institution: | (College of Enology,Northwest A & F University,Yangling 712100,Shaanxi) |
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| Abstract: | 【Objective】A total of 48 Saccharomyces cerevisiae strains from Xinjiang and Ningxia regions were typed by 4 microsatellite markers,and the genetic relations among the strains were also analyzed.【Method】A total of 48 Saccharomyces cerevisiae strains were discriminated for polymorphism at the 4 selected loci: SCAAT1,SCYOR267c,C11,and YPL009c.【Result】A total of 87 alleles,which generated 39 genotypes,were found.The polymorphism information contents(PIC) of all strains at 4 loci were 0.8593-0.9115,suggesting that the 4 SSR loci were hypervariable.The heterozygosity expected and heterozygosity observed were 0.8803-0.9268 and 0.8333-0.9792,respectively.【Conclusion】The results showed the diversity of Saccharomyces cerevisiae strains in Ningxia and Xinjiang regions. |
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| Keywords: | Saccharomyces cerevisiae microsatellite markers strain typing biodiversity |
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