| 基于SSR标记的甜瓜品种（系）DNA指纹图谱库的构建 |
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| 引用本文： | 宋海斌,崔喜波,马鸿艳,朱子成,栾非时.基于SSR标记的甜瓜品种（系）DNA指纹图谱库的构建[J].中国农业科学,2012,45(13):2676-2689. |
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| 作者姓名： | 宋海斌 崔喜波 马鸿艳 朱子成 栾非时 |
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| 作者单位： | 1.东北农业大学园艺学院，哈尔滨 150030;
2.黑龙江傲龙农业有限公司，哈尔滨150020 |
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| 基金项目： | 国家西甜瓜产业技术体系—分子育种岗位项目 |
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| 摘 要： | 【目的】建立甜瓜品种的DNA指纹图谱数据库，实现对甜瓜品种进行快速、准确的鉴定。【方法】应用SSR分子标记技术，首先利用20份具有代表性的甜瓜品种（系）筛选SSR引物，然后对105份不同甜瓜品种（系）进行指纹图谱的构建。【结果】从1 219对SSR引物中筛选出18对引物为105份材料形成了多态性的指纹图谱，其中每对引物可以检测到4—14条数目不等的多态性条带，平均为9条；多态性信息含量（PIC）平均为0.68，变化范围为0.55—0.82。105份材料间的相似系数为0.70—0.99。利用这18对SSR核心引物构建的指纹图谱库能够有效区分所有供试材料，并且为每份材料建立了一份独特的指纹图谱。【结论】SSR标记适于构建甜瓜品种 （系）的DNA指纹图谱库，可为甜瓜品种鉴定提供依据。
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| 关 键 词： | 甜瓜 指纹图谱 SSR标记 核心引物 |
| 收稿时间： | 2011-12-30 |
Construction of DNA Fingerprint Database Based on SSR Marker for Varieties(Lines) of Cucumis melo L |
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| SONG Hai-bin
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CUI Xi-bo
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MA Hong-yan
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ZHU Zi-cheng
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LUAN Fei-shi.Construction of DNA Fingerprint Database Based on SSR Marker for Varieties(Lines) of Cucumis melo L[J].Scientia Agricultura Sinica,2012,45(13):2676-2689. |
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| Authors: | SONG Hai-bin CUI Xi-bo MA Hong-yan ZHU Zi-cheng LUAN Fei-shi |
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| Institution: | 1(1College of Horticulture,Northeast Agricultural University,Harbin 150030;2Heilongjiang Aolong Agricultural Limited Company,Harbin 150020) |
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| Abstract: | 【Objective】DNA fingerprinting database of varieties of melon(Cucumis melo L.) was constructed and the identification method was obtained.【Method】The simple sequence repeat(SSR) primers were selected using 20 representative melon varieties(lines) for establishing fingerprints of 105 different varieties(lines).【Result】Out of the 1 219 pairs of SSR primers,18 pairs were screened to generate polymorphic fingerprints among 105 varieties or inbred lines.The results showed that each pair of the primers could detect 4-14 polymorphic bands with an average of 9.The average polymorphism information content(PIC) was 0.68 with a range of 0.55-0.82.The genetic similarity coefficient of the 105 accessions ranged from 0.70 to 0.99.The fingerprint data of all the 18 pairs of core SSR primers were employed to establish the fingerprinting database to distinguish the 105 samples and every accession had its unique fingerprinting.【Conclusion】The results indicated that SSR marker is suitable to construction of DNA fingerprinting database of melon varieties and the database could provide reference for melon identification. |
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| Keywords: | melon(Cucumis melo L ) fingerprinting SSR marker core primer |
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