Resistance to the cabbage root fly, Delia radicum, within Brassica fruticulosa |
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| Authors: | EB Jensen G Felkl K Kristiansen SB Andersen |
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| Institution: | (1) Institute of Nuclear Agricultural Sciences, Zhejiang University, Hua Jia Chi Campus, Hangzhou, 310029, China;(2) Institute of Entomology, Department of Plant Protection, Zhejiang University, Hua Jia Chi Campus, Hangzhou, 310029, China;(3) Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, KIN 6N5, Canada |
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| Abstract: | Two transgenic Bt rice lines, KMD1 and KMD2, both containing a synthetic cry1Ab gene from Bt, were crossed with conventional rice varieties. The inheritance of resistance to SSB of KMD1 and KMD2was investigated
through LSB and field examination of their progenies, e.g. F1, BC1 and F2 populations. In LSBs, 100.0% of newly hatched SSB larvae died on the second day after feeding on leaf tissues of F1 and GUS positive BC1 plants, of which the area of leaf tissues consumed by SSB is also similar to that of transgenic parents. These results imply
that the resistance of Bt rice to SSB is dominantly controlled and could be easily exploited in hybrid rice production. Field
evaluation showed that segregation ratios for SSB resistance to susceptibility in BC1 populations fit the ratio of 1:1, which was also confirmed by LSBs. However, in F2 populations, the ratio was significantly smaller than 3:1 for resistant to susceptible plants in all 6 indica × japonica
(KMD1 and KMD2) crosses, though it fitted 3:1 in all 4 japonica × japonica crosses. The results implied that the resistance
of Bt rice to SSB was controlled by a dominant gene which was present in a homozygous condition in both KMD1 and KMD2, but
the inheritance could be affected by other factors. Assays for Cry1Ab protein showed that, in most crosses, the content of
Cry1Ab is significantly higher in leaves of GUS positive F1, BC1 and F2 plants than that in transgenic Bt parent plants, which accounts for the high resistance observed in these plants to SSB.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | Bt Chilo suppressalis inheritance Oryzasativa L resistance transgenic |
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