| 自然生草改变苹果园土壤根际细菌群落的组成与结构 |
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| 引用本文： | 姚城城,张杰,卢艳芬,马孟霞,姚允聪.自然生草改变苹果园土壤根际细菌群落的组成与结构[J].北京农学院学报,2017,32(4). |
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| 作者姓名： | 姚城城 张杰 卢艳芬 马孟霞 姚允聪 |
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| 作者单位： | 北京农学院植物科学技术学院/农业应用新技术北京市重点实验室/北京林果业生态环境功能提升协同中心,北京,102206;北京农学院植物科学技术学院/农业应用新技术北京市重点实验室/北京林果业生态环境功能提升协同中心,北京,102206;北京农学院植物科学技术学院/农业应用新技术北京市重点实验室/北京林果业生态环境功能提升协同中心,北京,102206;北京农学院植物科学技术学院/农业应用新技术北京市重点实验室/北京林果业生态环境功能提升协同中心,北京,102206;北京农学院植物科学技术学院/农业应用新技术北京市重点实验室/北京林果业生态环境功能提升协同中心,北京,102206 |
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| 基金项目： | 国家重大科技专项课题，北京市重大科技项目 |
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| 摘 要： | 【目的】为了研究自然生草对果园土壤微生物群落的组成、结构及时空变化特征的影响,进而对增强果园土壤肥力、改善理化性状、促进果树生长发育方面产生的积极作用。【方法】利用Miseq高通量测序技术对自然生草(TR)与清耕(CK)条件下,根际土壤细菌群落进行测定与分析。【结果】结果表明,与清耕相比,自然生草明显提高果实膨大期土壤0~20cm、20~40cm的土层的有机质、全氮、碱解氮、速效磷及速效钾含量;降低土壤pH值。自然生草比清耕明显提高土壤细菌群落的丰度、种类丰富度和多样性指数(Shannon Index)。在门级水平上,宏基因组检测样本中共有37个门,变形菌门是比较明显的优势菌群,其次为酸杆菌门、浮霉菌门、放线菌门;在属分类级别上,宏基因组检测样本中共有470类菌属,芽单胞菌属(Gemmatimonas)、鞘氨醇单胞菌属(Sphingomonas)、类似牙球菌属Blastocatella是优势菌属。自然生草明显提高Ohtaekwangia、Chryseolinea的比例,降低Gaiella、化螺菌属(Nitrospira)比例;韦恩图显示细菌群落自然生草与清耕共有470类菌属,自然生草有63类独特的菌属,清耕有72类独特的菌属。【结论】自然生草提高苹果园土壤细菌群落的多样性,改良群落的结构和组成,明显提升土壤的养分状况。
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| 关 键 词： | 苹果园 自然生草 清耕 宏基因组测序 |
Natural grassimproves the composition and structure of the bacterial community in the soil of apple orchards |
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| YAO Chengcheng,ZHAG Jie,LU Yanfen,MA Mengxia,YAO Yuncong.Natural grassimproves the composition and structure of the bacterial community in the soil of apple orchards[J].Journal of Beijing Agricultural College,2017,32(4). |
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| Authors: | YAO Chengcheng ZHAG Jie LU Yanfen MA Mengxia YAO Yuncong |
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| Abstract: | Objective] Natural grass is an important method of soil management in our country.In order to study the influence of the orchard soil microbial community composition,structure and temporal variation characteristics,and then to enhance the orchard soil fertility,improve the physical and chemical properties,and promote the positive effect on fruit tree growth.Methods]Using Miseq high flux sequencing technology,the soil bacterial community were determined and analyzed on natural grass (TR) and clean tillage (CK) conditions.Results]Results showed that compared with clean tillage,natural grass significantly increased soil organic matter,total nitrogen,alkali solution nitrogen,available phosphorus and available potassium content and decreased the pH of soils in the layers of 0-20 cm and 20-40 cm in fruit maturity period.Natural grasses significantly improved the abundance,variety and diversity of soil bacterial communities (Shannon Index) compared with clean tillage.In phylum level,thirty seven bacteria phylum were detected in macrogenome sequencing,Proteobacteria is superiority,followed by Acidobacteria,Planctomycetes,and Actinobacteria.In the level of classification of bacteria genus,a total of 470 bacteria genus were detected in macrogenome sequencing,Gemmatimonas,Sphingomonas,and Planctomyces took clear superiority.Natural grasses significantly increased the proportion of Ohtaekwangia and Chryseolinea and decreased the proportion of Gaiella and Nitrospira.The winn diagram showed that there were 470 bacteria genus in the bacterial community of natural grasses and clean tillage,and the natural grasses have 63 unique bacteria genus,while there were 72 unique bacteria genus in clean tillage.Conclusion]Natural grass improved the diversity of the bacterial community in orchard soil,improved the structure and composition of the community,and obviously increased the nutrient status of the soil. |
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| Keywords: | apple orchards natural grass clean tillage macrogenome sequencing |
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