| 草莓果实中FaSAMDC基因的克隆、生物信息学及表达分析 |
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| 引用本文： | 于晓阳,沈元月.草莓果实中FaSAMDC基因的克隆、生物信息学及表达分析[J].北京农学院学报,2017,32(3). |
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| 作者姓名： | 于晓阳 沈元月 |
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| 作者单位： | 农业应用新技术北京市重点实验室/北京农学院植物科学技术学院,北京,102206;农业应用新技术北京市重点实验室/北京农学院植物科学技术学院,北京,102206 |
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| 基金项目： | 北京市自然科学基金资助项目，科技北京百名领军人才培养计划 |
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| 摘 要： | 【目的】为明确S-腺苷甲硫氨酸脱羧酶(SAMDC)在非呼吸跃变型草莓果实成熟中的作用。【方法】以‘北农香’草莓果实为试验材料,首先通过RT-PCR克隆FaSAMDC基因,其次通过SqRT-PCR分析发育果实(小绿、大绿、褪绿、白果、片红、全红)中的FaSAMDC基因的表达量。【结果】结果表明,FaSAMDC基因含有1 116bp开放阅读框,编码371个氨基酸,含有典型的脱羧酶保守功能结构域。随着草莓的果实发育,FaSAMDC表达量呈明显下降趋势并且在白果时期达到最低值,此后随着果实着色,表达量迅速上升并且成熟期达到最高。【结论】以上研究结果证实,S-腺苷甲硫氨酸脱羧酶可能参与草莓果实的成熟调控。
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| 关 键 词： | 草莓果实 S-腺苷甲硫氨酸脱羧酶 基因克隆 半定量分析 |
Cloning,bioinformatics and expression analysis of FaSAMDC gene in strawberry fruits |
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| YU xiaoyang,SHEN Yuanyue.Cloning,bioinformatics and expression analysis of FaSAMDC gene in strawberry fruits[J].Journal of Beijing Agricultural College,2017,32(3). |
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| Authors: | YU xiaoyang SHEN Yuanyue |
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| Abstract: | Objective] To clarify the role of S-adenosylmethionine decarboxylase (SAMDC) in fruit ripening of non-climacteric strawberry.Methods]The fruit of ‘Beinongxiang'strawberry fruit was used as material,the FaSAMDC gene was cloned by RT-PCR,and the mRNA expression levels of FaSAMDC gene in developmental fruits were studied by SqRT-PCR including the stages small green (SG),big green (BG),de-greening (DG),white (Wt),partial red (PR),full red (FR)].Results]The results showed that,FaSAMDC gene contains an open reading frame of 1 116 bp,encoding 371 amino acids,typically containing decarboxylase conserved functional domains.During the fruit development of strawberry,the expression of FaSAM-DC showed a decreasing trend and reached the lowest value in the white period;then,with the fruit coloring,the expression level increased rapidly and reached the highest level at full red stage.Conclusion]These results suggested that S-adenosylmethionine decarboxylase may be involved in the regulation of strawberry fruit ripening. |
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| Keywords: | strawberry fruit S-adenosylmethionine decarboxylase gene cloning Semi-quantitative analysis |
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