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梨轮纹病拮抗细菌的筛选与评价
引用本文:刘邮洲,常有宏,魏本强,刘永峰,陈志谊.梨轮纹病拮抗细菌的筛选与评价[J].果树学报,2009,26(3).
作者姓名:刘邮洲  常有宏  魏本强  刘永峰  陈志谊
作者单位:江苏省农业科学院植物保护研究所,南京,210014  
基金项目:江苏省农业科技自主创新资金项目 
摘    要:采集南京、连云港、高邮3地梨园病叶、病果、健叶、健果、病枝、健枝和土壤样本90份,分离纯化后得到871个细菌分离物,对其进行梨轮纹病菌菌丝生长的皿内抑制试验,共获得21株拮抗能力较强的细菌,其中拮抗性能最好的细菌sf 628抑菌圈直径达45 mm,抑制率达59.21%。梨果接种试验结果表明,21株拮抗能力较强的细菌中有5株拮抗细菌对梨轮纹病菌引起的病斑扩展抑制率大于50%,其中菌株sf 628抑制率达60.61%。进一步的理化特性和分子鉴定结果表明,菌株sf 628为枯草芽孢杆菌枯草亚种。

关 键 词:  拮抗细菌  筛选  抑制率  鉴定

Screening and evaluation of antagonistic bacteria against Botryosphaeria berengeriana f. sp. piricola
LIU You-zhou,CHANG You-hong,WEI Ben-qiang,LIU Yong-feng,CHEN Zhi-yi.Screening and evaluation of antagonistic bacteria against Botryosphaeria berengeriana f. sp. piricola[J].Journal of Fruit Science,2009,26(3).
Authors:LIU You-zhou  CHANG You-hong  WEI Ben-qiang  LIU Yong-feng  CHEN Zhi-yi
Institution:Institute of Plant Protection;Jiangsu Academy of Agricultural Sciences;Nanjing;Jiangsu 210014 China
Abstract:Biological control on plant diseases could reduce the residue of chemical pesticides and protect natural ecological environment.Pear ring rot is one of common diseases in pear production.The aim of this study was to screen and evaluate the antagonistic bacteria against Botryosphaeria berengeriana f.sp.piricola,which caused pear ring rot.871 strains were isolated and purified from the diseased leaves,the diseased pomes,the healthy leaves,the healthy pomes,the diseased tress,the healthy tress and the soil fro...
Keywords:Pear  Antagonistic bacteria  Screening  Inhibition rate  Identification  
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