| 用Nested-PCR检测广西黄皮与九里香黄龙病病原 |
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| 引用本文： | 邓崇岭 陈传武,赵小龙,付慧敏,陈国平, 白先进,娄兵海,吴礽超.用Nested-PCR检测广西黄皮与九里香黄龙病病原[J].中国农学通报,2010,26(17):273-276. |
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| 作者姓名： | 邓崇岭 陈传武 赵小龙 付慧敏 陈国平 白先进 娄兵海 吴礽超 |
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| 作者单位： | 1. 广西壮族自治区柑桔研究所,广西桂林,541004
2. 广西壮族自治区农业厅,南宁,530022 |
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| 基金项目： | 科技部国际科技合作项目"柑桔优势品种繁育及其高效栽培关键技术研究"，农业部公益性行业(农业)科研专项"柑桔模式化栽培与贮藏技术研究"，广西农业重点科技计划项目"柑桔黄龙病综合防治技术研究"，广西自然科学基金项目"黄皮、九里香抗柑桔黄龙病机理研究" |
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| 摘 要： | 摘要：运用nested-PCR技术对广西黄皮和九里香进行柑桔黄龙病（Citrus Huanglongbing, HLB）检测，结果表明：从部分黄皮和九里香样品抽提的DNA中，可扩增出一条特异性的400 bp条带，说明广西种植的黄皮和九里香均可感染黄龙病；在柑桔黄龙病疫区，黄皮和九里香黄龙病感染率分别为35.0%和40.0%。
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| 关 键 词： | 黄皮 九里香 黄龙病 Nested-PCR |
| 收稿时间： | 2010/4/21 0:00:00 |
| 修稿时间： | 2010/6/11 0:00:00 |
Citrus Huanglongbing Nested-PCR Detection of wampee and Murraya paniculata in Guangxi |
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| Deng Chongling,Chen Chuanwu,Zhao Xiaolong,Fu Huimin,Chen Guoping,Bai Xianjin,Lou Binghai,Wu Rengchao.Citrus Huanglongbing Nested-PCR Detection of wampee and Murraya paniculata in Guangxi[J].Chinese Agricultural Science Bulletin,2010,26(17):273-276. |
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| Authors: | Deng Chongling Chen Chuanwu Zhao Xiaolong Fu Huimin Chen Guoping Bai Xianjin Lou Binghai Wu Rengchao |
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| Institution: | 1Guangxi Citrus Research Institute, Guilin Guangxi 541004; 2Department of Agriculture of Guangxi, Nanning 530022) |
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| Abstract: | Nested-PCR was used for the detection of the pathogen of citrus Huanglongbing （HLB） on 40 samples ofClausena lansium andMurraya paniculata from several cities of Guangxi. The result demonstrated that a specific 400 bp band could be amplified from part of the DNA extracted from these plant materials, respectively. It indicated that Clausena lansium and Murraya paniculata from Guangxi could be infected by HLB. In HLB-infected area, HLB infection rates ofClausena lansium andMurraya paniculata were 35.0% and 40.0% , respectively. For the purpose of reduction of HLB infection rates and integrated control of HLB, Clausena lansium andMurraya paniculata should not be planted in the citrus planting area, and plants infected by HLB should be removed as soon as possible. |
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| Keywords: | Nested-PCR |
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