| 猪体细胞克隆技术的优化 |
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| 引用本文： | 张德福,戴建军,吴彩凤,吴华莉,刘东,陈学进,陈莹,王凯.猪体细胞克隆技术的优化[J].农业生物技术学报,2009,17(3):365-369. |
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| 作者姓名： | 张德福 戴建军 吴彩凤 吴华莉 刘东 陈学进 陈莹 王凯 |
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| 作者单位： | 1. 上海市农业科学院畜牧兽医研究所,上海201106;上海市农业遗传育种重点实验室,上海201106
2. 上海交通大学医学院附属新华医院发育生物学实验室,上海,200092 |
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| 基金项目： | 上海市科技兴农重点攻关项目，上海市科技兴农推广项目，上海市科学技术发展基金，德国BMBF |
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| 摘 要： | 摘要: 用体细胞核移植方法生产的克隆猪诞生至今已经8年，虽然同胚胎细胞核移植相比，体细胞核移植技术难度大，成功率低（1~2%），但近年来用体细胞核移植技术克隆猪有了较深入的研究，在体细胞核移植基本机理和关键技术上取得了一定的进展。本文结合本实验室近年来的相关研究工作，综述了用核移植方法生产体细胞克隆猪的技术关键和难点及应用情况，并对提高猪体细胞核移植效率的策略进行了分析。
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| 关 键 词： | 猪 猪体细胞核移植 优化 |
| 收稿时间： | 2008-6-25 |
| 修稿时间： | 2008-8-18 |
Optimization of Somatic Cell Nuclear Transfer in Pigs |
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| ZHANG De-fu,DAI Jian-jun,WU Cai-feng,WU Hua-li,LIU Dong,CHEN Xue-jin,CHEN Yin,WANG Kai.Optimization of Somatic Cell Nuclear Transfer in Pigs[J].Journal of Agricultural Biotechnology,2009,17(3):365-369. |
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| Authors: | ZHANG De-fu DAI Jian-jun WU Cai-feng WU Hua-li LIU Dong CHEN Xue-jin CHEN Yin WANG Kai |
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| Institution: | ZHANG De-fu1,2**,DAI Jian-jun1,2,WU Cai-feng1,WU Hua-li1,LIU Dong1,CHEN Xue-jin3,CHEN Yin3,WANG Kai3 (1.Animal Husbandry and Veterinary Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China,2. Shanghai Municipal Key Laboratory of Agri-genetics and Breeding,3. Laboratory of Biology Research Center,Xinhua Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200092,China ) |
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| Abstract: | Abstract: It has been almost eight years since the first report of live piglets produce by somatic cell nuclear transfer (SCNT) technology. Although it is more difficult, low efficiency (1~2%), compared with embryonic cell nuclear (ECNT) technology, the cloning pigs with SCNT technology, has been further studied. Some progress has taken in the fields of mechanism and approaches. In this paper, we mainly focus on the key technology and its difficulties of SCNT in cloning pigs, including its applications and approach of increasing the efficiency of SCNT. |
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| Keywords: | pig somatic cell nuclear transfer (SCNT) optimization |
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