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Evidence of Schmallenberg virus circulation in ruminants in Greece |
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| Authors: | Serafeim C Chaintoutis Evangelos Kiossis Nektarios D Giadinis Christos N Brozos Corinne Sailleau Cyril Viarouge Emmanuel Bréard Maria Papanastassopoulou Stéphan Zientara Orestis Papadopoulos Chrysostomos I Dovas |
| Institution: | 1. Laboratory of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, University Campus, 541 24, Thessaloniki, Greece 2. Clinic of Farm Animals, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 11 Stavrou Voutyra str, 546 27, Thessaloniki, Greece 3. UMR Virology, ANSES/INRA/ENVA, French Agency for Food, Environmental and Occupational Health and Safety, Maisons-Alfort, Alfort, 22 rue Pierre Curie, 947 03, Maisons-Alfort, France |
| Abstract: | During March 2013, we investigated the presence and the levels of Schmallenberg virus (SBV) circulation in three dairy cow herds and three sheep flocks in Central Macedonia, Greece. In two cow herds, a high number of abortions had been observed during the winter. Six bulk-tank milk samples and 147 individual sera were screened for SBV-specific antibodies by ELISA. Positive reactions were obtained from 5 out of 6 bulk-tank milk samples, 58 out of 90 sera from the 3 cow herds, and 2 sera from 2 of the 3 sheep flocks. Twenty-two ELISA-positive sera were tested by serum neutralization test (SNT). SNT confirmed the presence of neutralizing antibodies against SBV in all samples tested, with titers ranging between 1:32 and ≥1:256. No neutralizing antibodies against Akabane virus (AKAV) or Shamonda virus (SHAV) were detected, indicating that neutralizing antibodies against SBV do not cross react with AKAV or SHAV in SNT. ELISA testing of bulk-tank milk samples proved to be convenient and reliable. None of the tested sera was found positive for SBV by real-time RT-PCR, indicating that the sampling was conducted past the viremia stage. This is the first report of SBV circulation in Greece. |
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