Clinical and virological findings in pups naturally infected by canine parvovirus type 2 Glu-426 mutant. |
| |
| Authors: | Nicola Decaro Costantina Desario Marco Campolo Gabriella Elia Vito Martella Dominga Ricci Eleonora Lorusso Canio Buonavoglia |
| |
| Institution: | Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, Valenzano, Bari, Italy. |
| |
| Abstract: | An outbreak of canine parvovirus type 2 infection caused by the Glu-426 mutant in 2 litters of pups is reported. The infected pups (n = 6) were monitored daily for evidence of clinical signs and hematological changes and for the evaluation of viral shedding in the feces. The disease induced by the Glu-426 mutant was mild in all the infected pups. Vomiting and hemorrhagic diarrhea were not observed; however, the pups developed mucoid diarrhea (3.5 median days), depression (1.5 median days), and relative leukopenia and lymphopenia (2.5 median days). Fever and loss of appetite were observed only in 2 pups. Virus was detected in the feces for 4.5, 6.5, and 46 median days by hemagglutination, virus isolation on cell cultures, and real-time polymerase chain reaction (PCR), respectively. By real-time PCR, the highest viral DNA titers were detected in the feces of both litters at day 10, reaching median values of more than 10(10) DNA copies/mg of feces. |
| |
| Keywords: | |
|
|
