| 猪链球菌2型江苏分离株溶血素的纯化 |
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| 引用本文： | 方绍庆,陆承平.猪链球菌2型江苏分离株溶血素的纯化[J].中国预防兽医学报,2003,25(5):363-365. |
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| 作者姓名： | 方绍庆 陆承平 |
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| 作者单位： | 南京农业大学,农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 国家 973项目子课题 (G19990 1190 6 ) |
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| 摘 要： | 将猪链球菌2型(Streptococcus suis type2)江苏分离株HA9801接种于THB培养基中培养，得到含溶血素的培养液，其溶血价为128，再经50％饱和硫酸铵沉淀，脱盐，得到溶血素粗提物，溶血价为2048。粗提物用阴离子交换柱层析及凝胶过滤层析，所得活性峰收集液溶血价分别为4096、1024。通过以上三步的纯化，溶血素的比活提高200倍。纯化蛋白在SDS-PAGE中呈现一条带，达电泳级纯度。
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| 关 键 词： | 猪链球菌 溶血素 纯化 |
| 文章编号： | 1008-0589(2003)05-0363-03 |
| 修稿时间： | 2002年9月16日 |
Purification of haemolysin of Streptococcus suis type 2 strain HA9801 isolated in Jiangsu province |
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| FANG Shao-qing,LU Cheng-ping.Purification of haemolysin of Streptococcus suis type 2 strain HA9801 isolated in Jiangsu province[J].Chinese Journal of Preventive Veterinary Medicine,2003,25(5):363-365. |
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| Authors: | FANG Shao-qing LU Cheng-ping |
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| Institution: | FANG Shao-qing,LU Cheng-ping~* |
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| Abstract: | The haemolysin of streptococcus suis type 2 strain HA9801 isolated in Jiangsu province was purified from the culture supernatant,by using different methods,including selective(NH_4)_2SO_4 precipitation,desalting,anion-exchange chromatography,and sephacery1 chromatography,and showed the activity of 128,2048,4096,1024 hamolytic units respectively.The specific activity of the purified haemolysin was enhanced 1 528 folds.The purified hamelysin appeared a single band with molecular mass of 54 000 Da by SDS-PAGE.It sugguested the purified haemolysin of Jiangsu isolate was analogue of suilysin purified from U.S.A isolated previously reported. |
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| Keywords: | streptococcus suis haemolysin purification |
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