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对玉米螟具有生物活性的海洋放线菌筛选与鉴定
引用本文:刘志航,袁忠林,罗兰.对玉米螟具有生物活性的海洋放线菌筛选与鉴定[J].玉米科学,2015,23(2):147-151.
作者姓名:刘志航  袁忠林  罗兰
作者单位:青岛农业大学农学与植物保护学院/山东省植物病虫害综合防控重点实验室, 山东青岛266109,青岛农业大学农学与植物保护学院/山东省植物病虫害综合防控重点实验室, 山东青岛266109,青岛农业大学农学与植物保护学院/山东省植物病虫害综合防控重点实验室, 山东青岛266109
基金项目:国家级大学生创新创业训练计划(201210435002)、“十二五”农村领域国家科技计划课题(BAD19B04-09)、山东省高等学校青年骨干教师国内访问学者项目、山东省“泰山学者”建设工程专项、青岛农业大学第六届大学生创新教育立项经费
摘    要:通过平板稀释法对来自青岛不同地区的海洋样品进行分离,共得到18株海洋放线菌。采用浸虫法测定海洋放线菌发酵液对玉米螟2龄幼虫的室内毒力,筛选得到2株活性较高的菌株YC3和SLR3,其48 h发酵液对玉米螟2龄幼虫的校正死亡率分别为86.21%和81.04%。根据形态特征、培养特征及生理生化性状将活性菌株鉴定为链霉菌属(Streptomyces)。

关 键 词:玉米  放线菌  发酵液  玉米螟
收稿时间:2014/6/12 0:00:00

Screening and Identification of Marine Actinomyces with Bioactive for Asian Corn Borer(Ostrinia furnacalis G.)
LIU Zhi-hang,YUAN Zhong-lin and LUO Lan.Screening and Identification of Marine Actinomyces with Bioactive for Asian Corn Borer(Ostrinia furnacalis G.)[J].Journal of Maize Sciences,2015,23(2):147-151.
Authors:LIU Zhi-hang  YUAN Zhong-lin and LUO Lan
Institution:College of Agronomy and Plant Protection of Qingdao Agricultural University/Key Lab of Integrated Crop Pest Management of Shandong Province, Qingdao 266109, China,College of Agronomy and Plant Protection of Qingdao Agricultural University/Key Lab of Integrated Crop Pest Management of Shandong Province, Qingdao 266109, China and College of Agronomy and Plant Protection of Qingdao Agricultural University/Key Lab of Integrated Crop Pest Management of Shandong Province, Qingdao 266109, China
Abstract:Eighteen strain marine actinomyces were isolated from sea samples of Qingdao different regions by using dilution-plate method. Toxicities of fermentation broth from these marine actinomyces were assayed against 2 instar larvae of Asian corn borer by insect immersion bioassay method, and two strains had insecticidal activities for Ostrinia furnacalis G. The corrected mortality rates were 86.21% and 81.04% respectively in 48 h after treatment with supernatant from fermentation broth of YC3 and SLR3. The morphological and cultural characteristics, physiological and biochemical properties showed two strains were Streptomyces.
Keywords:Maize  Actinomyces  Fermentation broth  Ostrinia furnacalis G  
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