Anti-enzyme antibodies in rabbits and pigs against Hyostrongylus rubidus (Hassall and Stiles, 1892) |
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| Authors: | S Masaba IV Herbert |
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| Institution: | Department of Applied Zoology, University College of North Wales, Bangor, Gwynedd, Great Britain |
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| Abstract: | Ether extracts of Hyostrongylus rubidus adult worms isolated on days 14, 35 and 64 of intection were found to contain two isoenzymes of malic dehydrogenase (MDH) and three of acid phosphatase. Sera from rabbits immunized against these extracts and sera from pigs experimentally infected with H. rubidus were tested for their anti-enzyme activity by two different techniques.Sera from rabbits actively immunized with a Day 14 worm extract contained an antibody which complexed only with a slow migrating isoenzyme of acid phosphatase but not with isoenzymes of MDH or acetylcholinesterase (AChE). No antibodies against worm acid phosphates or MDH were detectable by this technique in the sera of pigs which were experimentally infected with H. rubidus.A different technique, however, where the worm extracts were incubated at 60°C either with rabbit anti-H. rubidus serum, or serum from infected pigs, indicated the presence of anti-AChE globulin in both immunized rabbits and infected pigs. When individual immunoglobulins isolated from infected pigs were incubated with the same worm extract it was seen that activity was associated with IgG1 but not with IgG2, IgM or IgA. IgG1 prepared from worm-free pigs did not complex with worm AchE. There was no interaction between the third stage larval AChE and pig IgG1. Levels of AChE were highest in worms isolated at a period of infection when the hosts immune responses were beginning to manifest themselves and lowest in those worms surviving the population crisis. |
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