| 高亲和铵转运因子在春小麦中的遗传转化研究 |
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| 引用本文： | 张宏纪,刘东军,孙岩,郭怡璠,刘文林,杨淑萍,宋凤英.高亲和铵转运因子在春小麦中的遗传转化研究[J].黑龙江农业科学,2014(6):1-4. |
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| 作者姓名： | 张宏纪 刘东军 孙岩 郭怡璠 刘文林 杨淑萍 宋凤英 |
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| 作者单位： | 黑龙江省农业科学院作物育种研究所,黑龙江哈尔滨150086 |
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| 基金项目： | 黑龙江省自然科学基金资助项目（C200830） |
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| 摘 要： | 为了培育可高效吸收利用铵态氮的小麦品种,将高亲和铵转运因子AtPRP3-AtAMT1;1融合基因导入优质强筋小麦品种龙辐18中,利用基因枪法对3 760个小麦幼胚愈伤组织进行了轰击。结果表明:经过筛选分化共获得69株抗性苗。用2对特异引物对转基因植株进行检测,成功获得4个转基因植株,转化率为0.11%,可作为选育高亲和铵转运因子小麦的原始材料。
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| 关 键 词： | 小麦 基因枪法 高亲和铵转运因子 |
Wheat Transformation with High-affinity NH4^＋ Transporter Gene by Particle Bombardment Method |
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| Institution: | Feng-ying (Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences, Harbin, Hei longjiang 150086) |
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| Abstract: | In order to breed wheat fusion gene was transformed into variety which could absorb NH4^＋ effectively,high-affinity NH4^＋ transporter immature embryo callus of wheat variety Longfumai 18 by particle bombardment method,after selection on media supplemented with different concentration of hygromycin, 69 resistant plants were regenerated from 3 760 immature embryo calluses. 4 transgenic plants were detected and confirmed by 2 pairs of specific primers. The average transformation frequency was 0.11%. It laid a solid foundation for breeding wheat with high efficiency NH＋ absorption. |
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| Keywords: | wheat particle bombardment method high-affinity NH4^＋ transporter gene |
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