| 一株海洋贝类肠道琼胶降解菌的筛选与鉴定 |
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| 引用本文： | 张林林,江玉姬,张龙涛,郑宝东,张怡,王培森,李庆旺.一株海洋贝类肠道琼胶降解菌的筛选与鉴定[J].福建农林大学学报(自然科学版),2016(1):89-93. |
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| 作者姓名： | 张林林 江玉姬 张龙涛 郑宝东 张怡 王培森 李庆旺 |
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| 作者单位： | 福建农林大学食品科学学院,福建 福州,350002 |
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| 基金项目： | 海洋经济创新发展区域示范项目(12PYY001SF08) |
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| 摘 要： | 以琼胶为唯一碳源,从海洋贝类肠道微生物共筛选出44株降解琼胶的海洋细菌,通过初筛和DNS法测定酶活得到一株产酶最高的菌株A-001,酶活力可达到13.01 U·m L-1.菌落形态为乳白色,革兰氏阴性菌,呈弯曲短杆状,通过微生物动力学实验,细菌A-001具有运动性.据生理生化特征和16Sr DNA序列可鉴定A-001为溶藻弧菌(Vibrio alginolyticus).
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| 关 键 词： | 贝类 琼胶酶 细菌 筛选 鉴定 |
Screening and identification of agarase-producing microbes in sea shellfish gut |
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| Abstract: | Agarase-producing microbes becomes hotspot for research and development on marine resources as agarase is mainly pro-duced by microbes in sea shellfish gut. Agarose was used as the only carbon source. Forty-four strains of agar-degrading bacteria were initially screened from marine shelfish gut. Then 6 strains, which presented bigger transparent circle around colony by Lugosi iodine liquid staining than the rest, were re-screened. Among them, strain A-001 showed the maximum enzyme activity at 13.01 U?mL-1 by DNS method. For the colony morphology, the strain A-001 was ivory, gram-negative and short rod. Through the dynamic experi-ment, the strain A-001 showed motility. Based on physiological and biochemical characteristics and 16SrDNA sequencing results, strain A-001 was identified as Vibrio alginolyticus. |
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| Keywords: | shellfish agarase bacteria screening identification |
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