Quantitative chromosome map of a representative indica rice |
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| Authors: | Somsak Apisitwanich Rieko Shishido Yukio Akiyama Kiichi Fukui |
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| Institution: | (1) Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand;(2) Department of Plant Science and Resources, College of Bioresource Science, Nihon University, 1866 Kamsino, Fujisawa Kanagawa 252-8510, Japan;(3) Laboratory of Genetic Engineering, Hokuriku National Agricultural Experiment Station, Joetsu 943-01, Japan;(4) Department of Biotechnology, Faculty of Engineering, Graduate School of Osaka University, Yamada-Oka 2-1, Suita 565-0871, Japan |
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| Abstract: | The somatic chromosomes of standard indica diploid rice, IR 36, were squashed on glass slides and stained with Giemsa. The condensation patterns (CP) of prometaphase
chromosomes were quantitatively analysed using CHIAS III software. The relative length and centromeric index (CI) were converted
from CHIAS III to numerical data and calculated by EXCEL program. The ideogram based on CP of indica rice was established. There were 2 pairs of satellite chromosomes and the result was confirmed by fluorescence in situ hybridization using 45S rDNA as a probe.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | indica rice IR 36 karyotyping Oryza sativa |
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