| 猪白细胞介素-15基因的克隆及生物信息学分析 |
| |
| 引用本文： | 骆爱芳,郭万柱,宋振辉,韩国全.猪白细胞介素-15基因的克隆及生物信息学分析[J].中国畜牧兽医,2007,34(10):37-40. |
| |
| 作者姓名： | 骆爱芳 郭万柱 宋振辉 韩国全 |
| |
| 作者单位： | 四川农业大学动物生物技术中心，雅安 625014 |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划);教育部长江学者和创新团队发展计划 |
| |
| 摘 要： | 参照GenBank登录的猪白细胞介素-15(IL-15)基因序列自行设计1对特异性引物，运用RT PCR技术从由刀豆蛋白A(ConA)诱导培养的荣昌猪外周血淋巴细胞扩增出猪IL-15基因的完整CDS序列，全长489 bp。同源比对结果表明，荣昌猪IL-15基因与已公布的2个猪种IL-15基因核苷酸同源性均为99.4%,与哺乳动物的同源性较高，与鸡的同源性较低。密码子偏爱性分析显示，荣昌猪IL-15基因在密码子使用上存在一定的偏爱性；分子进化分析结果表明其与人及哺乳动物IL-15基因的进化关系较近，而与禽类鸡的IL-15基因进化距离较远；结构域预测分析显示其与人的IL-15存在很大的相似性，可能在功能上有相似性。
|
| 关 键 词： | 荣昌猪 猪IL-15基因 克隆 生物信息学分析 |
| 文章编号： | 1671-7236（2007）10-0037-03 |
| 修稿时间： | 2007-04-02 |
Cloning and Phylogenetic Analysis of Interleukin-15 Gene in Rongchang Pig |
| |
| LUO Ai-fang,GUO Wan-zhu,SONG Zhen-hui,HAN Guo-quan.Cloning and Phylogenetic Analysis of Interleukin-15 Gene in Rongchang Pig[J].China Animal Husbandry & Veterinary Medicine,2007,34(10):37-40. |
| |
| Authors: | LUO Ai-fang GUO Wan-zhu SONG Zhen-hui HAN Guo-quan |
| |
| Institution: | Animal Biology Technology Center, Sichuan Agricultural University, Yaan 625014,China |
| |
| Abstract: | Using stimulated with ConA peripheral blood lymphocyte and a set of specific primers,the Interleukin-15 gene of swine was amplified by PCR.The primers were designed based on the published sequences of the swine IL-15 gene.The 489bp amplified DNA fragment was cloned into pMD18-T vetor.The results showed that the sequence identities were 99 between amplified IL15 gene and that from GenBank.Bioinformatics analysis indicated some Codon preference,Phylogenetic tree analysis indicated that the have evolutionary relationship with mammalian IL-15,however showed distinct difference with chicken IL-15.There are distinct resemblance between pig IL-15 and human IL-15 by structural domain prediction analysis. |
| |
| Keywords: | rongchang pig pig IL-15 cloning bioinformatics analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
