| 醋酸甲孕酮（MPA）间接竞争ELISA检测方法的建立 |
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| 引用本文： | 高艳兵,叶绍辉,龚振明.醋酸甲孕酮（MPA）间接竞争ELISA检测方法的建立[J].中国畜牧兽医,2007,34(10):124-126. |
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| 作者姓名： | 高艳兵 叶绍辉 龚振明 |
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| 作者单位： | 1.云南农业大学动物科学技术学院,昆明 650201；2.上海交通大学农业与生物学院，上海 201101 |
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| 摘 要： | 将半抗原醋酸甲孕酮（MPA）衍生物与牛血清白蛋白(BSA)偶联后，作为包被原与游离的醋酸甲孕酮竞争抗MPA多克隆抗体，通过该模式建立间接竞争ELISA方法检测MPA的残留量。试验结果表明：理想的包被抗原为0.625 μg/ml,抗体的工作浓度为1∶8000，标准曲线在1 ～50 ng/ml之间线性关系较好，最低检测限0.1 ng/ml, 得回归方程y=-1.1479x+0.9426(r2=0.9679),批内和批间变异系数分别为5. 35%、10. 89%。
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| 关 键 词： | 间接ELISA 半抗原 标准曲线 |
| 文章编号： | 1671-7236（2007）10-0124-03 |
| 修稿时间： | 2007-04-12 |
Establishment of Indirect Competitive Enzyme-linked Immunosorbent Assay for Detecting the Residues of MPA |
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| GAO Yan-bing,YE Shao-hui,GONG Zhen-ming.Establishment of Indirect Competitive Enzyme-linked Immunosorbent Assay for Detecting the Residues of MPA[J].China Animal Husbandry & Veterinary Medicine,2007,34(10):124-126. |
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| Authors: | GAO Yan-bing YE Shao-hui GONG Zhen-ming |
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| Institution: | 1.College of Animal Science, Yunnan Agricultural University, Kunming 650201,China; 2.School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 201101,China |
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| Abstract: | Coupled with the BSA, hapten MPA, used as a coating antigen，combined with anti-MPA polyclonal antibody with the competition of free MPA. Indirect competition ELISA was set up according to above mentioned mode to detect the residue level of MPA in the example. The results of the experiment demonstrates that the optimal concentration of the coating antigen was 0.75 μg/ml. Polyclonal antibody against MPA was 1∶8000 and the standard curve had a good linearity in the rang of 1~50 ng/ml with detection limit being 0.1 ng/ml. The regression equation y=1.1479x+0.9426(r2=0.9679) were obtained while the coefficient of variation of intra assay and extra-assay were 5.35%,10.89% respectively. |
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| Keywords: | ciELISA half antigen standard curve |
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