| 木薯渣固态发酵植酸酶的条件研究 |
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| 引用本文： | 马艳,洪葵,李枚秋,赵江涛.木薯渣固态发酵植酸酶的条件研究[J].热带作物学报,2000,21(2):58-64. |
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| 作者姓名： | 马艳 洪葵 李枚秋 赵江涛 |
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| 作者单位： | 华南热带农业大学工学院食品工程系，海南儋州571737 |
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| 摘 要： | 木薯渣粗蛋白及无机盐含量较低，与麸皮、米糠混合或同时添加无机氮源及无机盐，可作为发酵培养基合成植酸酶。高浓度的磷抑制植酸酶的合成。最佳发酵条件为：木薯渣为唯一碳原，添加 ２％ ＮＨ４ＮＯ３， ０．０１％ Ｋ２ＨＰＯ４， ０．０５％ ＭｇＳＯ４·７Ｈ２Ｏ， ０．０５％ ＫＣＩ， ０．０１％ ＦｅＳ－Ｏ４· ７Ｈ２Ｏ， ０．９％ ＳＤＳ，培养基水分含量 ６０％～６５％， １０ｇ木薯渣／２５０ ｍＬ三角瓶，接种量为２ｍＬ菌悬液（２．８×１０个孢子／ｍＬ），自然 ｐＨ值，培养温度 ２８～３１℃，第８ｄ达产酶高峰，高峰期产酶量为 ６７３０ Ｕ／ｇ干曲。
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| 关 键 词： | 植酸酶 木薯渣 固态发酵 |
Phytase from Solid Fermentation of Cassava Dregs |
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| Ma Yan,Hong Kui,Li Meiqiu,Zhao Jiangtao.Phytase from Solid Fermentation of Cassava Dregs[J].Chinese Journal of Tropical Crops,2000,21(2):58-64. |
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| Authors: | Ma Yan Hong Kui Li Meiqiu Zhao Jiangtao |
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| Institution: | Food Science and Technology Department, South China University of Tropical Agriculture, Danzhou, Hainan571737 |
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| Abstract: | Cassava dregs contained low crude protein and inorganic salt but could be used as a medium to produce phytase when added with wheat or rice bran or inorganic nitrogen and inorganic salt. Phytase production was inhibited by high level of phosphate. Optimizing fermentation conditions for phytase production were that cassava dregs, the only raw material, were added with mineral salts of2%nh4no3,0.01%k2PO4,0.05%gS4.7H2,0.05 KCl, 0.01 FeS0..7H, and 0.9 SDS, inoculated with mL (2x10* spore/mL) spore suspension/10 g cassava dregs of Aspergillus niger PD, and incubated at 28-31 C.The medium contained 10 g cassava dregs/250 mL conical flask and 60 %-65 %moisture with natural pH. The maximum yield 6 730 U/g dry koji of phytase was obtained at the eighth day. |
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| Keywords: | phytase cassava dregs solid fermentation |
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