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| 春兰菌根真菌的筛选 | |
| 引用本文： | 伍建榕,金辉,韩素芬,朱有勇,吕梅,王光萍.春兰菌根真菌的筛选[J].福建林学院学报,2007,27(3):267-271. |
| 作者姓名： | 伍建榕 金辉 韩素芬 朱有勇 吕梅 王光萍 |
| 作者单位： | 1. 西南林学院西南生物多样性保育国家林业局重点实验室,云南,昆明,650224 2. 南京林业大学森林资源与环境学院,江苏,南京,210037 3. 云南农业大学植物病理重点实验室,云南,昆明,650201 |
| 基金项目： | 国家自然科学基金，云南省教育厅资助项目 |
| 摘 要： | 地生兰组织培养存在移栽无菌苗成活率低、生长缓慢、开花迟缓、花小甚至不开花等问题,这些与缺少共生的菌根真菌有关。用分离自云南保山、大理等地野生春兰菌根中的内生真菌19个菌株接种春兰组培幼苗,经4.5个月的共生培养,兰苗叶色正常,根系生长良好。测定植物生长量,从长势有明显提高的接菌苗进行重分离及菌根的显微结构观察,确定春兰菌根的形成,并筛选出春兰的有效共生菌根真菌为CLB111,CLB113和MLX102。通过接种处理后春兰苗的鲜重增长率分别为70.6%、70.2%、68.6%,而不接菌的对照仅为45.6%,与对照差异达0.01极显著水平。结果表明这3个菌株均为春兰的菌根真菌的优良菌株. |
| 关 键 词： | 春兰 菌根真菌 接种 筛选 |
| 文章编号： | 1001-389X（2007）03-0267-05 |
| 收稿时间： | 2007-01-08 |
| 修稿时间： | 2007-01-082007-04-20 |
Screening mycorrhizal fungi from Cymbidium goeringii |
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| WU Jian-rong,JIN Hui,HAN Su-fen,ZHU You-yong,Lü Mei,WAN Guang-ping.Screening mycorrhizal fungi from Cymbidium goeringii[J].Journal of Fujian College of Forestry,2007,27(3):267-271. | |
| Authors: | WU Jian-rong JIN Hui HAN Su-fen ZHU You-yong Lü Mei WAN Guang-ping |
| Institution: | 1. Key Laboratory of Biodiversity Conservation in Southwest China of State Forestry Admlnistration, Southwest Forest College, Kunming, Yunnan 650224, China; 2. College of Forest Resources and Environment Nanjing Forest University, Nanjing, Jiangsu 210037, China; 3. The Phytopathology Laboraroty of Yunnan Agriculture University, Kunming, Yunnan 650201, China |
| Abstract: | The main problem in tissue culture of terrestrial orchids lies in low survival rate after transplantation,slow growth,delayed blossom and no blossom,which has something to do with lack of symbiont mycorrhizal fungi.The CLB111,CLB113(from wild Cymbidium goeringii and MLX102(from wild Cymbidium sinense) proved to be good mycorrhizal fungi strains for C.goeringii through inoculation,biomass measurement,re-isolation and micro-observation of root chip.The three strains mentioned above were inoculated into the roots of plantlets of C.goeringii.After four and half months of symbioses culture,plantlets showed a normal leave color,well developed root system.The fresh biomass of C.goeringii inoculated with CLB111,CLB113 and MLX102 increased by 70.6%,70.2% and 68.6% respectively while that of control is 45.6%.The statistics showed that the difference between inoculation group and control group was significant,the fresh weight increment of CLB111,CLB113 and MLX102 was greatly different from that of control(α=0.01). |
| Keywords: | Cymbidium goeringii mycorrhizal fungi inoculate screening |
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