| 茶籽苗儿茶素类、嘌呤碱及游离氨基酸分布规律的研究 |
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| 引用本文： | 陈林,宛晓春,张正竹,陈琪.茶籽苗儿茶素类、嘌呤碱及游离氨基酸分布规律的研究[J].茶叶科学,2008,28(1):43-49. |
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| 作者姓名： | 陈林 宛晓春 张正竹 陈琪 |
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| 作者单位： | 1. 安徽农业大学教育部农业部茶叶生物化学与生物技术重点实验室,安徽,合肥,230036;福建省农业科学院茶叶研究所,福建,福安,355015
2. 安徽农业大学教育部农业部茶叶生物化学与生物技术重点实验室,安徽,合肥,230036 |
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| 基金项目： | 国家自然科学基金
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国家重点基础研究发展规划(973计划) |
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| 摘 要： | 应用HPLC-PDAD和氨基酸自动分析仪对4～5叶初展的福云6号沙培茶籽苗各部位儿茶素类、嘌呤碱、氨基酸(茶氨酸)进行分析测定。结果表明:儿茶素类、嘌呤碱(咖啡碱、可可碱)主要分布于茶籽苗叶片及嫩茎,子叶下部含量明显降低;EGCG、茶氨酸在茶籽苗嫩叶中含量较高,并随叶片成熟不断减少,但茶氨酸以侧根和主根最为富集,其代谢前体氨基酸(Glu、Ala)在根部亦具较高含量。随着茶籽萌发,茶氨酸在胚根大量合成,初露紫红色嫩茎的茶籽苗根系茶氨酸含量达13.429mg/g鲜重。据此认为培育茶籽及其无性系幼苗,并以幼嫩根系为材料,有望成为茶籽综合利用及天然茶氨酸获取的新途径。
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| 关 键 词： | 茶籽苗 儿茶素 茶氨酸 咖啡碱 |
| 文章编号： | 1000-369X（2008）01-043-07 |
| 收稿时间： | 2007-05-29 |
| 修稿时间： | 2007-11-08 |
Distribution of Catechins, Purine Alkaloids and Free Amino Acids in Tea Seedlings |
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| CHEN Lin,WAN Xiao-chun,ZHANG Zheng-zhu,CHEN Qi.Distribution of Catechins, Purine Alkaloids and Free Amino Acids in Tea Seedlings[J].Journal of Tea Science,2008,28(1):43-49. |
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| Authors: | CHEN Lin WAN Xiao-chun ZHANG Zheng-zhu CHEN Qi |
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| Abstract: | Catechins,purine alkaloids and free amino acids in various parts of tea seedlings(Camellia sinensis cv.Fuyun 6) with four or five leaves were analyzed by HPLC-PDAD and amino acid analyzer.The results showed that catechins and purine alkaloids mainly existed in the leaves and tender stems,and less in the parts below cotyledons.There were higher(-)-epigallocatechin gallate and L-theanine contents in the tender leaves,which decreased as they grew up.L-theanine was especially abundant in the roots of tea seedlings,the same to its precursors,glutamic acid and alanine.L-theanine was largely formed in radicles during germination of tea seeds,and reached 13.429 mg/g of fresh weight when purplish red colored stems of tea seedlings came out.Thus,it would be a promising way to obtain natural L-theanine from the tender roots of tea seedlings and its clones. |
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| Keywords: | tea seedlings catechins caffeine L-theanine |
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