| 铁皮石斛促生内生真菌的筛选与鉴定 |
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| 引用本文： | 吴慧凤,宋希强,胡美姣.铁皮石斛促生内生真菌的筛选与鉴定[J].西南林学院学报,2011(5):47-52. |
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| 作者姓名： | 吴慧凤 宋希强 胡美姣 |
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| 作者单位： | 海南大学热带作物种质资源保护与开发利用教育部重点实验室;海南大学园艺园林学院;中国热带农业科学院环境与植物保护研究所; |
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| 基金项目： | 科技部科技人员服务企业项目(2009GJE20004)资助; 教育部博士点基金项目(20104601110002)资助; 海南大学植物学重点学科项目资助; 热带作物种质资源保护与开发利用教育部重点实验室开放基金资助 |
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| 摘 要： | 从铁皮石斛根中分离得到37株内生真菌，经过与组培苗共生筛选，获得9株促生菌，其中3株真菌对铁皮石斛的鲜质量增长率、根数增长率和芽数的促生效果最好。采用形态学观察和ITS序列测定相结合的方法对这9株促生菌进行鉴定。经形态学观察，初步鉴定出2株，均为瘤菌根菌属。根据rDNAITS序列分析显示，2株与Epulorhizasp．的相似性分别为100％和96％，1株与Guignardiacamelliae的相似性达100％，1株与MycosphaereUacruenta的相似性为99％。另外5株促生真菌分类地位尚不能确定，但这5株菌株与登录号为EU482263、GQ996178、AM901831、GU566294和GU566256的序列相似性分别达96％、95％、99％、98％和100％。
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| 关 键 词： | 铁皮石斛 内生真菌 分子鉴定 |
Screening and Identification of Endophytic and Probiotic Fungi of Dendrobium catenatum |
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| WU Hui-feng,SONG Xi-qiang,HU Mei-jiao.Screening and Identification of Endophytic and Probiotic Fungi of Dendrobium catenatum[J].Journal of Southwest Forestry College,2011(5):47-52. |
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| Authors: | WU Hui-feng SONG Xi-qiang HU Mei-jiao |
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| Institution: | WU Hui-feng1,SONG Xi-qiang1,2,HU Mei-jiao3(1.Key Laboratory of Tropical Crop Germplasm Resource Conservation and Utilization,Hainan University,Ministry of Education,Danzhou Hainan,571737,China,2.College of Horticulture and Landscape Architecture,3.Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,China) |
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| Abstract: | 37 strains of endophytic fungi were isolated from roots of Dendrobium catenatum.Among which 9 strains were screened out to show the positive effect on the growth after being in vitro inoculated with D.catenatum seedlings.3 strains were the most beneficial promote the growth rate of fresh biomass,the increment rate of the radical number,and the number of new buds.The 9 probiotic fungi were identified by morphological characteristics observation and ITS gene sequence analysis.2 strains were identified to belo... |
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| Keywords: | Dendrobium catenatum endophytic fungi molecular identification |
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