| 22个苹果品种和砧木试管苗对热处理脱毒效应的研究 |
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| 引用本文： | 晏娜,孙建设,邵建柱,高仪,李玲.22个苹果品种和砧木试管苗对热处理脱毒效应的研究[J].河北农业大学学报,2009,32(5). |
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| 作者姓名： | 晏娜 孙建设 邵建柱 高仪 李玲 |
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| 作者单位： | 河北农业大学,园艺学院,河北,保定,071001 |
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| 基金项目： | 河北省科技厅资助项目，农业部公益性行业科研专项，农业部国家现代苹果产业技术体系功能岗位专家项目 |
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| 摘 要： | 用RT-PCR对22个苹果品种和砧木携带3种潜隐性病毒的情况进行了检测,并用变温热处理结合茎尖培养对22个苹果品种和砧木组培苗脱除潜隐性病毒的效应进行了研究。结果表明:不同的苹果品种和砧木耐热性不同。其中‘王林’、‘乔纳金’、‘嘎啦’、‘金冠’、‘珠美海棠’、‘P22’的耐热性最强;‘GM256’、‘弘前’、‘SH’系耐热性最差;‘惠民短枝富士’、‘浓红富士’、‘天红1号’、‘B9’、‘M9’、‘P59’、‘77-34’、‘天红2号’、‘Jazz’的耐热性居中。在相同处理条件下,不同的苹果基因型脱毒率存在差异。‘惠民短枝富士’、‘SH17’、‘M9’、‘GM256’、‘弘前’脱毒率最高,‘77-34’、‘P59’、‘P22’、‘天红1号’次之,‘乔纳金’、‘SH40’、‘SH38’、‘浓红富士’的脱毒率最低。另外,不同病毒种类脱除的难易程度也存有差异,ACLSV最容易脱除,ASGV最难脱除,ASPV居中。
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| 关 键 词： | 苹果品种 砧木 苹果潜隐性病毒 热处理 脱毒 |
Responses of twenty-two apple cultivars and rootstocks to thermotherapy for virus elimination in vitro |
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| YAN Na,SUN Jian-she,SHAO Jian-zhu,GAO Yi,LI Ling.Responses of twenty-two apple cultivars and rootstocks to thermotherapy for virus elimination in vitro[J].Journal of Agricultural University of Hebei,2009,32(5). |
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| Authors: | YAN Na SUN Jian-she SHAO Jian-zhu GAO Yi LI Ling |
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| Institution: | YAN Na,SUN Jian-she,SHAO Jian-zhu,GAO Yi,LI Ling(College of Horticulture,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | 3 latent viruses in 22 apple cultivars and rootstocks were detected by RT-PCR technology,and their responses to alternating heat therapy in combination with shoot tip culture for virus elimination in vitro were studied.The results showed that the heat resistance was different among these 22 apple cultivars and rootstocks,of which 'Orin','Jonagold','Gala','Golden Delicious','Zumi' and 'P22' exhibited the highest heat resistance,'Huimin spur Fuji','Deep red Fuji','Tianhong No.1','B9','M9','P59','77-34','Tianh... |
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| Keywords: | apple cultivars rootstocks latent virus heat treatment virus eliminationment virus elimination |
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