| 河豚毒素两种定量检测方法的比较研究 |
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| 引用本文： | 李世平,焦新安,黄金林,潘志明,王丽丽,何蕊.河豚毒素两种定量检测方法的比较研究[J].扬州大学学报(农业与生命科学版),2004,25(2):58-60. |
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| 作者姓名： | 李世平 焦新安 黄金林 潘志明 王丽丽 何蕊 |
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| 作者单位： | 扬州大学,生物科学与技术学院,江苏,扬州,225009 |
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| 基金项目： | 国家“十五”科技攻关项目(2001BA804A14)，教育部“高校青年教师奖”(175)，江苏省“六大人才高峰”项目 |
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| 摘 要： | 应用小鼠生物试验和间接竞争抑制酶联免疫吸附试验(ELISA)同步检测17份河豚鱼肝组织和20份河豚鱼肌肉组织中的河豚毒素含量，并对2种方法进行比较。结果表明：ELISA法与小鼠生物试验测得的结果相符合。ELISA法由于其测定程序简便易行，速度快，灵敏度高，在河豚毒素的定量检测以及在预防河豚鱼中毒方面具有广泛的应用前景。
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| 关 键 词： | 河豚毒素 间接竞争抑制酶联免疫吸附试验 小鼠生物试验 |
| 文章编号： | 1671-4652(2004)02-0058-03 |
| 修稿时间： | 2004年3月10日 |
Comparison of Two Methods of Quantitatively Detecting Tetrodotoxin |
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| LI Shi-ping,JIAO Xin-an,HUANG Jin-lin,PAN Zhi-ming,WANG Li-li,HE Rui.Comparison of Two Methods of Quantitatively Detecting Tetrodotoxin[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2004,25(2):58-60. |
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| Authors: | LI Shi-ping JIAO Xin-an HUANG Jin-lin PAN Zhi-ming WANG Li-li HE Rui |
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| Abstract: | The concentrations of tetrodotoxin in 17 livers and 20 muscles of puffer fishes were detected by mouse bioassay and indirect competitive inhibition enzyme-linked immunosorbent assay (ELISA) respectively. The results showed that ELISA was remarkably correlated to mouse bioassay. Because ELISA is an easy-handled, rapid and highly sensitive method, it would play an important role in quantitatively detecting tetrodotoxin and effectively regulating poisonous puffer fishes. |
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| Keywords: | tetrodotoxin indirect competitive inhibition enzyme-linked immunosorbent assay mouse bioassay |
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